Abstract | BACKGROUND: Diagnostic tests for fish allergy are hampered by the large number of under-investigated fish species. Four salmon allergens are well-characterized and registered with the WHO/IUIS while no catfish allergens have been described so far. In 2008, freshwater-cultured catfish production surpassed that of salmon, the globally most-cultured marine species. We aimed to identify, quantify, and compare all IgE- binding proteins in salmon and catfish. METHODS: Seventy-seven pediatric patients with clinically confirmed fish allergy underwent skin prick tests to salmon and catfish. The allergen repertoire of raw and heated protein extracts was evaluated by immunoblotting using five allergen-specific antibodies and patients' serum followed by mass spectrometric analyses. RESULTS: CONCLUSIONS: We detail the allergen repertoire of two highly farmed fish species. IgE-binding to fish tropomyosins and TPIs was demonstrated for the first time in a large patient cohort. Tropomyosins, in addition to parvalbumins, should be considered for urgently needed improved fish allergy diagnostics.
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Authors | Thimo Ruethers, Aya C Taki, Shaymaviswanathan Karnaneedi, Shuai Nie, Tanja Kalic, Danyi Dai, Sakda Daduang, Michael Leeming, Nicholas A Williamson, Heimo Breiteneder, Sam S Mehr, Sandip D Kamath, Dianne E Campbell, Andreas L Lopata |
Journal | Allergy
(Allergy)
Vol. 76
Issue 5
Pg. 1443-1453
(05 2021)
ISSN: 1398-9995 [Electronic] Denmark |
PMID | 32860256
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2020 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd. |
Chemical References |
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Topics |
- Allergens
(immunology)
- Animals
- Catfishes
- Child
- Food Hypersensitivity
(diagnosis)
- Humans
- Parvalbumins
- Salmon
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