Retinol palmitate against toxicogenic damages of antineoplastic drugs on normal and tumor cells.

Abstract

The lack of tissue selectivity of anticancer drugs generates intense collateral and adverse effects of cancer patients, making the incorporation of vitamins or micronutrients into the diet of individuals to reduce side or adverse effects of antineoplastics. The study aimed to evaluate the effects of retinol palmitate (RP) on the toxicogenic damages induced by cyclophosphamide (CPA), doxorubicin (DOX) and its association with the AC protocol (CPA + DOX), in Sarcoma 180 (S-180) tumor cell line, using the micronuclei test with a block of cytokinesis (CBMN); and in non-tumor cells derived from Mus musculus using the comet assay. The results suggest that CPA, DOX and AC protocol induced significant toxicogenic damages (P < 0.05) on the S-180 cells by induction of micronuclei, cytoplasmic bridges, nuclear buds, apoptosis, and cell necrosis, proving their antitumor effects, and significant damage (P < 0.001) to the genetic material of peripheral blood cells of healthy mice, proving the genotoxic potential of these drugs. However, RP modulated the toxicogenic effects of antineoplastic tested both in the CBMN test (P < 0.05), at the concentrations of 1, 10 and 100 IU/mL; as in the comet assay (P < 0.001) at the concentration of 100 IU/kg for the index and frequency of genotoxic damage. The accumulated results suggest that RP reduced the action of antineoplastics in non-tumor cells as well as the cytotoxic, mutagenic, and cell death in neoplastic cells.

• Authors: Ricardo Melo de Carvalho, Marcus Vinicius Oliveira Barros de Alencar, Ana Maria Oliveira Ferreira da Mata, Rosália Maria Tôrres de Lima, Rai Pablo Sousa de Aguiar, Jadson Silva Teixeira, Márcia Fernanda Correia Jardim Paz, Soane Kaline Morais Chaves, Muhammad Torequl Islam, João Marcelo de Castro E Sousa, Paulo Michel Pinheiro Ferreira, Ana Amélia de Carvalho Melo Cavalcante, Bahare Salehi, William N Setzer, Javad Sharifi-Rad
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 330 Pg. 109219 (Oct 01 2020) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 32846153 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Antineoplastic Agents
• Diterpenes
• Retinyl Esters
• Vitamin A
• retinol palmitate
• Doxorubicin
• Cyclophosphamide

Topics

• Animals
• Antineoplastic Agents (toxicity)
• Antineoplastic Combined Chemotherapy Protocols (adverse effects, toxicity)
• Apoptosis (drug effects)
• Cell Line
• Cell Line, Tumor
• Comet Assay
• Cyclophosphamide (adverse effects, toxicity)
• DNA Damage (drug effects)
• Diterpenes (pharmacology)
• Dose-Response Relationship, Drug
• Doxorubicin (adverse effects, toxicity)
• Drug Interactions
• Humans
• Mice
• Micronucleus Tests
• Mutagenesis (drug effects)
• Retinyl Esters
• Vitamin A (analogs & derivatives, pharmacology)