Abstract |
Mutation in rpsL (encoding ribosomal protein S12), rrs (encoding 16S ribosomal RNA) and gidB (encoding 7- methylguanosine methyltransferase) are associated with resistance to streptomycin (STR), which is used for the treatment of multi-drug resistant tuberculosis (MDR-TB) in Nepal. The aim of our study is to analyze the correlation between mutations in the target genes and STR-resistance in 197 Mycobacterium tuberculosis (MTB) isolates from Nepal. Mutations in rpsL was harbored by 65.9% of isolates, in which the most common mutation in rpsL is caused by K43R (58.8%) and were significantly associated with Beijing genotype (P < 0.001). About 13.2% of isolates harbored mutations in two highly mutable regions of rrs, the 530 loop and the 912 region. About 13.2% of gidB mutants do not show any mutation in rpsL and rrs, which might suggest the role of gidB mutations in STR-resistance in MTB. In addition, 5.6% of isolates do not show any mutations in three genes examined, suggesting the involvement of other mechanism in STR-resistance in MTB. Our findings can be implemented for the establishment of molecular STR-susceptibility testing, in which tuberculosis can be treated with appropriate drugs and can improve control strategies for DR-TB.
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Authors | Dipti Shrestha, Bhagwan Maharjan, Nan Aye Thida Oo, Norikazu Isoda, Chie Nakajima, Yasuhiko Suzuki |
Journal | Tuberculosis (Edinburgh, Scotland)
(Tuberculosis (Edinb))
Vol. 125
Pg. 101985
(12 2020)
ISSN: 1873-281X [Electronic] Scotland |
PMID | 32829153
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 Elsevier Ltd. All rights reserved. |
Chemical References |
- Anti-Bacterial Agents
- DNA, Bacterial
- RNA, Ribosomal, 16S
- Ribosomal Proteins
- ribosomal protein S12
- Streptomycin
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Topics |
- Anti-Bacterial Agents
(pharmacology)
- DNA Mutational Analysis
- DNA, Bacterial
(genetics)
- Drug Resistance, Bacterial
(genetics)
- Genotype
- Humans
- Incidence
- Microbial Sensitivity Tests
- Mutation
- Mycobacterium tuberculosis
(genetics)
- Nepal
(epidemiology)
- RNA, Ribosomal, 16S
(genetics, metabolism)
- Retrospective Studies
- Ribosomal Proteins
(genetics, metabolism)
- Streptomycin
(pharmacology)
- Tuberculosis, Multidrug-Resistant
(epidemiology, genetics, microbiology)
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