Nicotinamide-induced mouse embryo developmental defect rescued by resveratrol and I-CBP112.

Abstract

Cell cycle of mouse embryo could be delayed by nicotinamide (NAM). Histone H3 lysine 56 (H3K56ac) acetylation plays an important role in mammalian genomic stability and the function of this modification in mouse embryos is not known. Hence, we designed to study the effects of NAM-induced oxidative stress on the developmental ability of mouse embryos, on the acetylation of H3K56ac and the possible functions of this modification related to mouse embryo development. Treatment with NAM (10, 20, or 40 mmol/L for 24 or 48 hr) during in vitro culture significantly decreased developmental rate of blastocyst (24 hr: 90.2 vs. 81.2, 43.2, and 18.2, with p > .05, p < .01, respectively; 48 hr: 89.3 vs. 53.2%, 12.1%, and 0% with p < .05, respectively). NAM treatment (20 mmol/L) for 6 and 31 hr resulted in increased intracellular reactive oxygen species levels in two-cell embryos, and apoptotic cell numbers in blastocysts. Resveratrol (RSV) and I-CBP112 rescued the 20 mmol/L NAM-induced embryo developmental defects. RSV and I-CBP112 increased the level of Sirt1 and decreased the level of H3K56ac induced by NAM in two-cell embryos (p < .05). These data suggest that NAM treatment decreases the expression of Sirt1, which induces high levels of H3K56 acetylation that may be involved in oxidative stress-induced mouse embryo defects, which can be rescued by RSV and I-CBP112.

• Authors: Yu-Guo Yuan, Jia-Lin Wang, Ayman Mesalam, Ling Li, Yun-Jung Choi, Abu Musa Md Talimur Reza, Dongjie Zhou, Li Chen, Chen Qian
• Journal: Molecular reproduction and development (Mol Reprod Dev) Vol. 87 Issue 9 Pg. 1009-1017 (09 2020) ISSN: 1098-2795 [Electronic] United States
• PMID: 32818292 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Wiley Periodicals LLC.

Chemical References

• Histones
• I-CBP112
• Oxazepines
• Piperidines
• Reactive Oxygen Species
• Niacinamide
• Sirtuin 1
• Resveratrol

Topics

• Animals
• Cells, Cultured
• Cytoprotection (drug effects, genetics)
• Embryo Culture Techniques
• Embryo, Mammalian (cytology, drug effects, metabolism)
• Embryonic Development (drug effects, genetics)
• Gene Expression Regulation, Developmental (drug effects)
• Histones (drug effects, metabolism)
• Mice
• Niacinamide (pharmacology)
• Oxazepines (pharmacology)
• Oxidative Stress (drug effects, genetics)
• Piperidines (pharmacology)
• Reactive Oxygen Species (metabolism)
• Resveratrol (pharmacology)
• Sirtuin 1 (genetics, metabolism)