Development of a marker vaccine candidate against classical swine fever based on the live attenuated vaccine C-strain.

Abstract

Classical swine fever (CSF) is a highly contagious and economically damaging disease. Classical swine fever virus (CSFV) lapinized vaccine C-strain against CSF worldwide lacks the capacity for the serological differentiation between infected and vaccinated animals (DIVA). To develop a marker C-strain complying with the DIVA principle, we generated and evaluated mutants rHCLV-E2F117A, rHCLV-E2G119A, and rHCLV-E2P122A, which harbor the single amino acid mutation at 117F, 119G or 122P of the monoclonal antibody HQ06-recognized epitope on the E2 glycoprotein in rabbits and pigs. Viral intravenous administration demonstrated that all the mutants retain the phenotype of C-strain in rabbits, including fever response induction and replication in the spleen. Notably, the HQ06-recognized epitope did not react with the antibodies induced by rHCLV-E2P122A in rabbits, in contrast with C-strain and other two mutants. Intramuscular administration of rHCLV-E2P122A in pigs induced anti-CSFV neutralizing antibodies but not antibodies against the HQ06-recognized epitope at 28 days post-inoculation. Collectively, our data demonstrate that rHCLV-E2P122A is a promising marker vaccine candidate against CSF.

• Authors: Yuying Han, Libao Xie, Mengqi Yuan, Yuteng Ma, Huimin Sun, Yuan Sun, Yongfeng Li, Hua-Ji Qiu
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 247 Pg. 108741 (Aug 2020) ISSN: 1873-2542 [Electronic] Netherlands
• PMID: 32768202 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies, Neutralizing
• Antibodies, Viral
• Vaccines, Attenuated
• Viral Vaccines

Topics

• Animals
• Antibodies, Neutralizing (blood, immunology)
• Antibodies, Viral (blood, immunology)
• Cell Line
• Classical Swine Fever (immunology, prevention & control)
• Classical Swine Fever Virus (genetics, immunology)
• HEK293 Cells
• Humans
• Immunogenicity, Vaccine
• Mutation
• Phenotype
• Rabbits
• Swine
• Swine Diseases (immunology, prevention & control, virology)
• Vaccination
• Vaccines, Attenuated (administration & dosage, immunology)
• Viral Vaccines (administration & dosage, genetics, immunology)