Analysis of opticin binding to collagen fibrils identifies a single binding site in the gap region and a high specificity towards thin heterotypic fibrils containing collagens II, and XI or V/XI.

Abstract

Opticin is a class III member of the extracellular matrix small leucine-rich repeat protein/proteoglycan (SLRP) family found in vitreous humour and cartilage. It was first identified associated with the surface of vitreous collagen fibrils and several other SLRPs are also known to bind collagen fibrils and it some cases alter fibril morphology. The purpose of this study was to investigate the binding of opticin to the collagen II-containing fibrils found in vitreous and cartilage. Electron microscopic studies using gold labelling demonstrated that opticin binds vitreous and thin cartilage collagen fibrils specifically at a single site in the gap region of the collagen D-period corresponding to the e2 stain band; this is the first demonstration of the binding site of a class III SLRP on collagen fibrils. Opticin did not bind thick cartilage collagen fibrils from cartilage or tactoids formed in vitro from collagen II, but shows high specificity for thin, heterotypic collagen fibrils containing collagens II, and XI or V/XI. Vitreous collagen fibrils from opticin null and wild-type mice were compared and no difference in fibril morphology or diameter was observed. Similarly, in vitro fibrillogenesis experiments showed that opticin did not affect fibril formation. We propose that when opticin is bound to collagen fibrils, rather than influencing their morphology it instead hinders the binding of other molecules to the fibril surfaces and/or act as an intermediary bridge linking the collagen fibrils to other non-collagenous molecules.

• Authors: Uwe Hansen, David F Holmes, Peter Bruckner, Paul N Bishop
• Journal: PloS one (PLoS One) Vol. 15 Issue 8 Pg. e0234672 ( 2020) ISSN: 1932-6203 [Electronic] United States
• PMID: 32764753 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Extracellular Matrix Proteins
• Optc protein, mouse
• Proteoglycans
• Collagen

Topics

• Animals
• Binding Sites
• Cattle
• Collagen (chemistry, metabolism, ultrastructure)
• Extracellular Matrix Proteins (chemistry, deficiency, metabolism)
• In Vitro Techniques
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Microscopy, Electron, Transmission
• Microscopy, Immunoelectron
• Protein Binding
• Proteoglycans (chemistry, deficiency, metabolism)
• Vitreous Body (chemistry, metabolism, ultrastructure)