Catalytic hairpin assembly-based electrochemical biosensor with tandem signal amplification for sensitive microRNA assay.

Abstract

We demonstrate for the first time the construction of a low background electrochemical biosensor with tandem signal amplification for sensitive microRNA assay based on target-activated catalytic hairpin assembly (CHA) of heteroduplex-templated copper nanoparticles. This electrochemical biosensor exhibits high sensitivity, good specificity, single-base mismatch discrimination capability, excellent stability and reproducibility, and it can sensitively detect microRNA in cancer cells.

• Authors: Lin Cui, Jinghua Zhou, Xiao-Yun Yang, Jing Dong, Xiaolei Wang, Chun-Yang Zhang
• Journal: Chemical communications (Cambridge, England) (Chem Commun (Camb)) Vol. 56 Issue 70 Pg. 10191-10194 (Sep 01 2020) ISSN: 1364-548X [Electronic] England
• PMID: 32748919 (Publication Type: Journal Article)

Chemical References

• MicroRNAs

Topics

• Base Pair Mismatch
• Biocatalysis
• Biosensing Techniques (methods)
• Cell Line, Tumor
• Electrochemistry
• Humans
• Inverted Repeat Sequences
• Limit of Detection
• MicroRNAs (genetics, metabolism)