Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses.

Abstract

Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

• Authors: K Reddisiva Prasanth, Minato Hirano, W Samuel Fagg, Eileen T McAnarney, Chao Shan, Xuping Xie, Adam Hage, Colette A Pietzsch, Alexander Bukreyev, Ricardo Rajsbaum, Pei-Yong Shi, Mark T Bedford, Shelton S Bradrick, Vineet Menachery, Mariano A Garcia-Blanco
• Journal: Antiviral research (Antiviral Res) Vol. 182 Pg. 104874 (10 2020) ISSN: 1872-9096 [Electronic] Netherlands
• PMID: 32735900 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Chemical References

• TOP3B protein, human
• DNA Topoisomerases, Type I

Topics

• Betacoronavirus (physiology)
• Cell Line
• DNA Topoisomerases, Type I (metabolism)
• Dengue Virus (physiology)
• Ebolavirus (physiology)
• Gene Knockout Techniques
• Humans
• Influenza A virus (physiology)
• RNA Viruses (metabolism)
• SARS-CoV-2
• Virus Replication (physiology)
• Yellow fever virus (physiology)
• Zika Virus (physiology)