Choice of Lipid Emulsion Determines Inflammation of the Gut-Liver Axis, Incretin Profile, and Insulin Signaling in a Murine Model of Total Parenteral Nutrition.

Abstract

SCOPE:

The aim of this study is to test whether the choice of the lipid emulsion in total parenteral nutrition (TPN), that is, n-3 fatty acid-based Omegaven versus n-6 fatty acid-based Intralipid, determines inflammation in the liver, the incretin profile, and insulin resistance.

METHODS AND RESULTS:

Jugular vein catheters (JVC) are placed in C57BL/6 mice and used for TPN for 7 days. Mice are randomized into a saline group (saline infusion with oral chow), an Intralipid group (IL-TPN, no chow), an Omegaven group (OV-TPN, no chow), or a chow only group (without JVC). Both TPN elicite higher abundance of lipopolysaccharide binding protein in the liver, but only IL-TPN increases interleukin-6 and interferon-γ, while OV-TPN reduces interleukin-4, monocyte chemoattractant protein-1, and interleukin-1α. Insulin plasma concentrations are higher in both TPN, while glucagon and glucagon-like peptide-1 (GLP-1) were higher in IL-TPN. Gluconeogenesis is increased in IL-TPN and the nuclear profile of key metabolic transcription factors shows a liver-protective phenotype in OV-TPN. OV-TPN increases insulin sensitivity in the liver and skeletal muscle.

CONCLUSION:

OV-TPN as opposed to IL-TPN mitigates inflammation in the liver and reduces the negative metabolic effects of hyperinsulinemia and hyperglucagonemia by "re-sensitizing" the liver and skeletal muscle to insulin.

• Authors: Phing-How Lou, Eliana Lucchinetti, Paulina Wawrzyniak, Yasser Morsy, Marcin Wawrzyniak, Michael Scharl, Stefanie D Krämer, Gerhard Rogler, Martin Hersberger, Michael Zaugg
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 65 Issue 5 Pg. e2000412 (03 2021) ISSN: 1613-4133 [Electronic] Germany
• PMID: 32729969 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Wiley-VCH GmbH.

Chemical References

• Emulsions
• Fatty Acids, Omega-6
• Fish Oils
• Incretins
• Insulin
• Interleukin-6
• Lipids
• Phospholipids
• Triglycerides
• fish oil triglycerides
• interleukin-6, mouse
• soybean oil, phospholipid emulsion
• Soybean Oil
• Interferon-gamma

Topics

• Animals
• Emulsions (administration & dosage, chemistry, pharmacology)
• Fatty Acids, Omega-6 (pharmacology)
• Fish Oils (pharmacology)
• Gastritis (etiology)
• Hepatitis (etiology)
• Incretins (metabolism)
• Insulin (blood, metabolism)
• Insulin Resistance
• Interferon-gamma (metabolism)
• Interleukin-6 (metabolism)
• Lipids (administration & dosage, chemistry)
• Malabsorption Syndromes (etiology)
• Male
• Mice, Inbred C57BL
• Muscle, Skeletal (drug effects, metabolism)
• Parenteral Nutrition, Total (adverse effects, methods)
• Phospholipids (administration & dosage, pharmacology)
• Soybean Oil (administration & dosage, pharmacology)
• Triglycerides (pharmacology)