Abstract |
To better understand the link between obesity and prostate cancer (PC) aggressiveness, we investigate the role of leptin, an obesity associated adipokine, and its receptor (Ob-R) in PC cells migration. The migration assay (Wound-healing) was used to study the leptin impact on DU-145 and PC3 cells lines. STAT3 activation was performed by Western Blot. E-cadherin expression was studied using fluorescence microscopy and Ob-R expression in PC and benign prostatic Hyperplasia (BPH) biopsies was assessed by RT-PCR. In this study we demonstrate that high dose of leptin promotes PC cells migration and EMT transition via the stimulation of STAT3 pathway. In addition, we report that although Ob-R mRNA is expressed by ADK and BPH resections biopsies, significant higher levels were observed for ADK patients. Finally, we found a positive association between Ob-R mRNA expression and worse PC prognosis. A better understanding of the molecular processes of leptin signaling is crucial for identifying appropriate approaches for treatment of obesity-related PC patients.
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Authors | Amal Gorrab, Alessandra Pagano, Khouloud Ayed, Mohamed Chebil, Amine Derouiche, Hervé Kovacic, Asma Gati |
Journal | Nutrition and cancer
(Nutr Cancer)
Vol. 73
Issue 7
Pg. 1217-1227
( 2021)
ISSN: 1532-7914 [Electronic] United States |
PMID | 32698628
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- LEP protein, human
- LEPR protein, human
- Leptin
- Receptors, Leptin
- STAT3 Transcription Factor
- STAT3 protein, human
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Topics |
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Humans
- Leptin
(pharmacology)
- Male
- Prostatic Neoplasms
(pathology)
- Receptors, Leptin
(genetics)
- STAT3 Transcription Factor
(genetics)
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