Shenmai Injection Supresses Glycolysis and Enhances Cisplatin Cytotoxicity in Cisplatin-Resistant A549/DDP Cells via the AKT-mTOR-c-Myc Signaling Pathway.

Abstract

Tumor cells, especially drug-resistant cells, predominately support growth by glycolysis even under the condition of adequate oxygen, which is known as the Warburg effect. Glucose metabolism reprogramming is one of the main factors causing tumor resistance. Previous studies on Shenmai injection (SMI), a Chinese herbal medicine, have shown enhanced efficacy in the treatment of tumors in combination with chemotherapy drugs, but the mechanism is not clear. In this study, we investigated the effect of SMI combined with cisplatin on cisplatin-resistant lung adenocarcinoma A549/DDP cells. Our results showed that cisplatin-resistant A549/DDP cells exhibited increased glucose consumption, lactate production, and expression levels of key glycolytic enzymes, including hexokinase 2 (HK2), pyruvate kinase M1/2 (PKM1/2), pyruvate kinase M2 (PKM2), glucose transporter 1 (GLUT1), and lactate dehydrogenase A (LDHA), compared with cisplatin-sensitive A549 cells. SMI combined with cisplatin in A549/DDP cells, led to significantly lower expression levels of key glycolytic enzymes, such as HK2, PKM1/2, GLUT1, and pyruvate dehydrogenase (PDH). In addition, we found that the combination of SMI and cisplatin could inhibit cell proliferation and promote apoptosis by reducing the expression levels of p-Akt, p-mTOR, and c-Myc, and then, it reduced the glycolysis level. These results suggest that SMI enhances the antitumor effect of cisplatin via glucose metabolism reprogramming. Therefore, the combination of SMI and cisplatin may be a potential therapeutic strategy to treat cisplatin-resistant nonsmall cell lung cancer.

• Authors: Ye Sun, Yushi Chen, Ming Xu, Chunying Liu, Hai Shang, Chun Wang
• Journal: BioMed research international (Biomed Res Int) Vol. 2020 Pg. 9243681 ( 2020) ISSN: 2314-6141 [Electronic] United States
• PMID: 32685545 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Ye Sun et al.

Chemical References

• Drug Combinations
• Drugs, Chinese Herbal
• MYC protein, human
• Proto-Oncogene Proteins c-myc
• fructus schizandrae, radix ginseng, radix ophiopogonis drug combination
• MTOR protein, human
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases
• Cisplatin

Topics

• A549 Cells
• Adenocarcinoma of Lung (drug therapy, metabolism, pathology)
• Cisplatin (pharmacology)
• Drug Combinations
• Drug Resistance, Neoplasm (drug effects)
• Drugs, Chinese Herbal (pharmacology)
• Glycolysis (drug effects)
• Humans
• Lung Neoplasms (drug therapy, metabolism, pathology)
• Proto-Oncogene Proteins c-akt (metabolism)
• Proto-Oncogene Proteins c-myc (metabolism)
• Signal Transduction (drug effects)
• TOR Serine-Threonine Kinases (metabolism)