The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.

Abstract

The emergence and rapid proliferation of the novel coronavirus (SARS-CoV-2) resulted in a global pandemic, with over 6,000,000 cases and nearly 400,000 deaths reported worldwide by the end of May 2020. A rush to find a cure prompted re-evaluation of a range of existing therapeutics vis-à-vis their potential role in treating COVID-19, placing a premium on analytical tools capable of supporting such efforts. Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity. Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein. Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor. Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers form 1:1 complexes with RBD, indicating the presence of a single binding site. This association alters the protein conformation (to maximize the contiguous patch of the positive charge on the RBD surface), resulting in a notable decrease in its ability to associate with ACE2. The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface. In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.

• Authors: Yang Yang, Yi Du, Igor A Kaltashov
• Journal: Analytical chemistry (Anal Chem) Vol. 92 Issue 16 Pg. 10930-10934 (08 18 2020) ISSN: 1520-6882 [Electronic] United States
• PMID: 32678978 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Gases
• Recombinant Proteins
• Spike Glycoprotein, Coronavirus
• spike protein, SARS-CoV-2
• Heparin
• Peptidyl-Dipeptidase A
• ACE2 protein, human
• Angiotensin-Converting Enzyme 2

Topics

• Angiotensin-Converting Enzyme 2
• Betacoronavirus (isolation & purification, metabolism)
• Binding Sites
• COVID-19
• Coronavirus Infections (drug therapy, pathology, virology)
• Gases (chemistry)
• Heparin (metabolism, pharmacology, therapeutic use)
• Humans
• Mass Spectrometry (methods)
• Molecular Dynamics Simulation
• Pandemics
• Peptidyl-Dipeptidase A (chemistry, genetics, metabolism)
• Pneumonia, Viral (drug therapy, pathology, virology)
• Protein Binding
• Protein Domains
• Recombinant Proteins (biosynthesis, chemistry, isolation & purification)
• SARS-CoV-2
• Spike Glycoprotein, Coronavirus (chemistry, metabolism)
• Virus Internalization (drug effects)