Abnormal epiphyseal development in a feline model of Sandhoff disease.

Abstract

Sandhoff disease (SD) is caused by decreased function of the enzyme β-N-acetylhexosaminidase, resulting in accumulation of GM2 ganglioside in tissues. Neural tissue is primarily affected and individuals with the infantile form of the disease generally do not survive beyond 4 years of age. Current treatments address neurometabolic deficits to improve lifespan, however, this extended lifespan allows clinical disease to become manifest in other tissues, including the musculoskeletal system. The impact of SD on bone and joint tissues has yet to be fully determined. In a feline model of infantile SD, animals were treated by intracranial injection of adeno-associated virus vectors to supply the central nervous system with corrective levels of hexosaminidase, resulting in a twofold to threefold increase in lifespan. As treated animals aged, signs of musculoskeletal disease were identified. The present study characterized bone and joint lesions from affected cats using micro-computed tomography and histology. All affected cats had similar lesions, whether or not they were treated. SD cats displayed a significant reduction in metaphyseal trabecular bone and markedly abnormal size and shape of epiphyses. Abnormalities increased in severity with age and appear to be due to alteration in the function of chondrocytes within epiphyseal cartilage, particularly the articular-epiphyseal complex. Older cats developed secondary osteoarthritic changes. The changes identified are similar to those seen in humans with mucopolysaccharidoses. Statement of clinical significance: the lesions identified will have significant implications on the quality of life of individuals whose lifespans are extended due to treatments for the primary neurological effects of SD.

• Authors: Margaret A McNulty, Patricia B Prevatt, Elizabeth R Nussbaum, Ashley N Randle, Aime K Johnson, Judith A Hudson, Heather L Gray-Edwards, Miguel Sena-Esteves, Douglas R Martin, Cathy S Carlson
• Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society (J Orthop Res) Vol. 38 Issue 12 Pg. 2580-2591 (12 2020) ISSN: 1554-527X [Electronic] United States
• PMID: 32678923 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Orthopaedic Research Society. Published by Wiley Periodicals LLC.

Topics

• Animals
• Cats
• Disease Models, Animal
• Genetic Therapy
• Growth Plate (diagnostic imaging, growth & development, pathology, physiopathology)
• Sandhoff Disease (diagnostic imaging, pathology, physiopathology, therapy)
• X-Ray Microtomography