Effect and mechanism of ginsenoside Rg1-regulating hepatic steatosis in HepG2 cells induced by free fatty acid.

Abstract

Ginsenoside Rg1 (G-Rg1) is a bioactive phytochemical that has been found to be beneficial for the treatment of several diseases including nonalcoholic fatty liver disease (NAFLD). But there is a lack of literature reporting the effect of G-Rg1 on lipid metabolism balance in NAFLD. We investigated the effect and mechanism of G-Rg1 on lipid metabolism in vitro. We found that G-Rg1 decreased the levels of TG, TC, and MDA, and increased activity of SOD. Results of RT-PCR and western blotting showed that supplementation with G-Rg1 downregulated the expression of PPAR γ, FABP1, FATP2/5, CD36, SREBP1 c, and FASN, while the expression of PPAR ɑ, CPT1, ACOX1, MTTP, and ApoB100 was upregulated, after induction by a free fatty acid. Taken together, we conclude that G-Rg1 inhibits lipid synthesis and lipid uptake, and enhances lipid oxidation and lipid export to reduce hepatic steatosis of HepG2 cells by regulating PPAR ɑ and PPAR γ expression.

• Authors: Yue Gao, Shujun Zhang, Jiajun Li, Jinqiu Zhao, Qing Xiao, Yali Zhu, Jia Zhang, Wenxiang Huang
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 84 Issue 11 Pg. 2228-2240 (Nov 2020) ISSN: 1347-6947 [Electronic] England
• PMID: 32654591 (Publication Type: Journal Article)

Chemical References

• Fatty Acids, Nonesterified
• Ginsenosides
• PPAR alpha
• Malondialdehyde
• ginsenoside Rg1

Topics

• Biological Transport (drug effects)
• Cell Survival (drug effects)
• Fatty Acids, Nonesterified (pharmacology)
• Gene Expression Regulation (drug effects)
• Ginsenosides (pharmacology, therapeutic use)
• Hep G2 Cells
• Humans
• Malondialdehyde (metabolism)
• Non-alcoholic Fatty Liver Disease (drug therapy, metabolism, pathology)
• PPAR alpha (metabolism)