Endocytosis is a pathway used by viruses to enter cells that can be classified based on the
proteins involved, such as
dynamin,
clathrin or
caveolin. Although the entry of
herpes simplex type 1 (HSV-1) by endocytosis has been documented in different cell types, its dependence on
clathrin has not been described whereas its dependence on
dynamin has been shown according to the cell line used. The present work shows how
clathrin-mediated endocytosis (CME) is one way that HSV-1 infects the human oligodendroglial (HOG) cell line. Partial
dynamin inhibition using
dynasore revealed a relationship between decrease of
infection and
dynamin inhibition, measured by viral titration and immunoblot. Co-localization between
dynamin and HSV-1 was verified by immunofluorescence at the moment of viral entry into the cell. Inhibition by
chlorpromazine revealed that viral progeny also decreased when
clathrin was partially inhibited in our cell line. RT-qPCR of immediately early viral genes, specific entry assays and electron microscopy all confirmed
clathrin's participation in HSV-1 entry into HOG cells. In contrast,
caveolin entry assays showed no effect on the entry of this virus. Therefore, our results suggest the participation of
dynamin and
clathrin during endocytosis of HSV-1 in HOG cells.