HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

A Comprehensive Proteomic SWATH-MS Workflow for Profiling Blood Extracellular Vesicles: A New Avenue for Glioma Tumour Surveillance.

Abstract
Improving outcomes for diffuse glioma patients requires methods that can accurately and sensitively monitor tumour activity and treatment response. Extracellular vesicles (EV) are membranous nanoparticles that can traverse the blood-brain-barrier, carrying oncogenic molecules into the circulation. Measuring clinically relevant glioma biomarkers cargoed in circulating EVs could revolutionise how glioma patients are managed. Despite their suitability for biomarker discovery, the co-isolation of highly abundant complex blood proteins has hindered comprehensive proteomic studies of circulating-EVs. Plasma-EVs isolated from pre-operative glioma grade II-IV patients (n = 41) and controls (n = 11) were sequenced by Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS) and data extraction was performed by aligning against a custom 8662-protein library. Overall, 4054 proteins were measured in plasma-EVs. Differentially expressed proteins and putative circulating-EV markers were identified (adj. p-value < 0.05), including those reported in previous in-vitro and ex-vivo glioma-EV studies. Principal component analysis showed that plasma-EV protein profiles clustered according to glioma histological-subtype and grade, and plasma-EVs resampled from patients with recurrent tumour progression grouped with more aggressive glioma samples. The extensive plasma-EV proteome profiles achieved here highlight the potential for SWATH-MS to define circulating-EV biomarkers for objective blood-based measurements of glioma activity that could serve as ideal surrogate endpoints to assess tumour progression and allow more dynamic, patient-centred treatment protocols.
AuthorsSusannah Hallal, Ali Azimi, Heng Wei, Nicholas Ho, Maggie Yuk Ting Lee, Hao-Wen Sim, Joanne Sy, Brindha Shivalingam, Michael Edward Buckland, Kimberley Louise Alexander-Kaufman
JournalInternational journal of molecular sciences (Int J Mol Sci) Vol. 21 Issue 13 (Jul 03 2020) ISSN: 1422-0067 [Electronic] Switzerland
PMID32635403 (Publication Type: Journal Article)
Chemical References
  • Biomarkers, Tumor
Topics
  • Adult
  • Aged
  • Biomarkers, Tumor (blood)
  • Brain Neoplasms (blood, classification, pathology)
  • Case-Control Studies
  • Cell-Derived Microparticles (metabolism, ultrastructure)
  • Cohort Studies
  • Extracellular Vesicles (metabolism, ultrastructure)
  • Female
  • Glioma (blood, classification, pathology)
  • Humans
  • Liquid Biopsy (methods)
  • Male
  • Middle Aged
  • Proteomics (methods)
  • Tandem Mass Spectrometry (methods)
  • Workflow

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: