miR-143-3p inhibits proliferation and invasion of hepatocellular carcinoma cells by regulating its target gene FGF1.

Abstract

PURPOSE:

To explore FGF1 and miR-143-3p expression in hepatocellular carcinoma (HCC) cells and its related mechanisms.

METHODS:

Eighty-two HCC patients treated at our hospital from January 2018 to January 2019 were enrolled as Group A, while further 80 healthy people undergoing physical examinations during the same time period were enrolled as Group B. HCC cells and normal human liver cells were purchased, with HepG2 and SMMC-7721 cells transfected with pcDNA3.1-FGF1, si-FGF1, NC, miR-143-3p-inhibitor and miR-143-3p-mimics. FGF1 and miR-143-3p expression was detected by qRT-PCR. The expression of N-cadherin, vimentin, Snail, Slug, E-cadherin and γ-catenin was detected by Western Blotting (WB). Cell proliferation was detected by MTT assay. Cell invasion was detected by Transwell. Cell apoptosis was detected by flow cytometry (FCM).

RESULTS:

FGF1 was highly expressed but miR-143-3p was poorly expressed in HCC cells. Areas under the curves (AUCs) of the two indicators were > 0.8. The indicators were correlated with the age, gender, tumor invasion, degree of differentiation, tumor location and TNM staging of the patients. Silencing FGF1 and overexpressing miR-143-3p could promote cell apoptosis, inhibit cell growth, cell epithelial-mesenchymal transition (EMT) and the expression of N-cadherin, vimentin, Snail and Slug, and increase the expression of E-cadherin and γ-catenin. Dual luciferase reporter gene assay (DLRGA) confirmed that FGF1 and miR-143-3p had a targeted relationship. The rescue experiment showed that the proliferation, invasion and apoptosis of HepG2 and SMMC-7721 cells in the miR-143-3p-mimics+pcDNA3.1-FGF1 and miR-143-3p-inhibitor+Si-FGF1 groups were not different from those in the miR-NC group.

CONCLUSION:

Inhibiting FGF1 can upregulate miR-143-3p-mediated Hedgehog signaling pathway, and affect cells' EMT, proliferation and invasion, so FGF1 is expected to become a potential therapeutic target for HCC.

• Authors: J Peng, H J Wu, H F Zhang, S Q Fang, R Zeng
• Journal: Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico (Clin Transl Oncol) Vol. 23 Issue 3 Pg. 468-480 (Mar 2021) ISSN: 1699-3055 [Electronic] Italy
• PMID: 32617870 (Publication Type: Journal Article)

Chemical References

• Cadherins
• MIRN143 microRNA, human
• MicroRNAs
• RNA Probes
• SNAI1 protein, human
• Snail Family Transcription Factors
• Vimentin
• gamma Catenin
• Fibroblast Growth Factor 1

Topics

• Age Factors
• Apoptosis
• Area Under Curve
• Cadherins (metabolism)
• Carcinoma, Hepatocellular (metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Epithelial-Mesenchymal Transition
• Female
• Fibroblast Growth Factor 1 (genetics, metabolism)
• Flow Cytometry
• Gene Silencing
• Humans
• Liver (cytology)
• Liver Neoplasms (metabolism, pathology)
• Male
• MicroRNAs (antagonists & inhibitors, metabolism)
• Middle Aged
• Neoplasm Invasiveness
• RNA Probes
• Sex Factors
• Snail Family Transcription Factors (metabolism)
• Vimentin (metabolism)
• gamma Catenin (metabolism)