Amyloids are a group of
proteins that are capable of forming aggregated
amyloid fibrils, which is responsible for many
neurodegenerative diseases including
Alzheimer's disease (AD). In our previous study, synthesis and characterization of star-shaped
poly(D,L-lactide)-b-
gelatin (ss-pLG) have been reported. In the present work, we have extended our work to study ss-pLG against
protein aggregation. To the best of our knowledge, this is the first report on the inhibition of
amyloid fibrillation by
protein grafted
poly(D,L-lactide).
Bovine serum albumin (BSA) was chosen as the model
protein, which readily forms fibril under high temperature. We found that ss-pLG efficiently suppressed the fibril formation of BSA compared with
gelatin (Gel), which was supported by
Thioflavin T assay, circular dichroism (CD) spectroscopy and atomic force microscopy (AFM). In addition, ss-pLG significantly curtailed
amyloid-induced
hemolysis. We also found that incubation of ss-pLG with
neuroblastoma cells (MC65) protected the cells from fibril-induced toxicity. The rescuing efficiency of ss-pLG was better than Gel, which could be attributed to the reduced lamella thickness in branched ss-pLG. These results suggest the significance of
gelatin grafting, which probably allows
gelatin to interact with the key residues of the amyloidogenic core of BSA effectively.