Abstract |
pH is a critical indicator of bone physiological function and disease status; however, noninvasive and real-time sensing of bone pH in vivo has been a challenge. Here, we synthesized a bone pH sensor by labeling alendronate with the H+-sensitive dye fluorescein isothiocyanate (Aln- FITC). Aln- FITC showed selective affinity for hydroxyapatite (HAp) rather than other calcium materials. An in vivo biodistribution study showed that Aln- FITC can be rapidly and specifically delivered to rat bones after caudal vein injection, and the fluorescence lasted for at least 12 h. The fluorescence intensity of Aln- FITC binding to HAp linearly decreased when the pH changed from 6 to 12. This finding was further confirmed on bone blocks and perfused bone when the pH changed from 6.8 to 7.4, indicating unique pH-responsive characteristics in the bone microenvironment. Aln- FITC was then preliminarily applied to evaluate the changes in bone pH in a nude mouse acidosis model. Our results demonstrated that Aln- FITC might have the potential for minimally invasive and real-time in vivo bone pH sensing in preclinical studies of bone healing, metabolism, and cancer mechanisms.
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Authors | Yuzhou Li, Yiru Fu, He Zhang, Jinlin Song, Sheng Yang |
Journal | BioMed research international
(Biomed Res Int)
Vol. 2020
Pg. 4012194
( 2020)
ISSN: 2314-6141 [Electronic] United States |
PMID | 32550231
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Yuzhou Li et al. |
Chemical References |
- Durapatite
- Fluorescein-5-isothiocyanate
- Alendronate
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Topics |
- Alendronate
(analysis, chemistry, pharmacokinetics)
- Animals
- Bone and Bones
(chemistry, metabolism)
- Durapatite
(chemistry, metabolism)
- Fluorescein-5-isothiocyanate
(analysis, chemistry, pharmacokinetics)
- Humans
- Hydrogen-Ion Concentration
- Male
- Mice, Nude
- Monitoring, Physiologic
- Optical Imaging
- Rats
- Rats, Sprague-Dawley
- Tissue Distribution
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