Abstract | BACKGROUND: Dental pulp stem cells (DPSCs) have high proliferation and multi-differentiation capabilities that maintain their functionality after cryopreservation. In our previous study, we demonstrated that cryopreserved rat DPSCs improved diabetic polyneuropathy and that the efficacy of cryopreserved rat DPSCs was equivalent to that of freshly isolated rat DPSCs. The present study was conducted to evaluate whether transplantation of cryopreserved human DPSCs (hDPSCs) is also effective for the treatment of diabetic polyneuropathy. METHODS: hDPSCs were isolated from human impacted third molars being extracted for orthodontic reasons. Eight weeks after the induction of diabetes in nude mice, hDPSCs (1 × 105/limb) were unilaterally transplanted into the hindlimb skeletal muscle, and vehicle (saline) was injected into the opposite side as a control. The effects of hDPSCs were analyzed at 4 weeks after transplantation. RESULTS: CONCLUSIONS:
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Authors | Masaki Hata, Maiko Omi, Yasuko Kobayashi, Nobuhisa Nakamura, Megumi Miyabe, Mizuho Ito, Eriko Makino, Saki Kanada, Tomokazu Saiki, Tasuku Ohno, Yuka Imanishi, Tatsuhito Himeno, Hideki Kamiya, Jiro Nakamura, Shogo Ozawa, Ken Miyazawa, Kenichi Kurita, Shigemi Goto, Jun Takebe, Tatsuaki Matsubara, Keiko Naruse |
Journal | Stem cell research & therapy
(Stem Cell Res Ther)
Vol. 11
Issue 1
Pg. 236
(06 16 2020)
ISSN: 1757-6512 [Electronic] England |
PMID | 32546222
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Vascular Endothelial Growth Factor A
- Streptozocin
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Topics |
- Animals
- Cell Differentiation
- Cells, Cultured
- Dental Pulp
- Diabetes Mellitus, Experimental
(therapy)
- Diabetic Neuropathies
(therapy)
- Humans
- Mice
- Mice, Nude
- Rats
- Rats, Sprague-Dawley
- Stem Cells
- Streptozocin
- Vascular Endothelial Growth Factor A
(genetics)
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