To explore the pathogenesis of heart and kidney imbalance
insomnia and the regulatory effect of
Jiaotai Pills, in order to study the changes of central and peripheral
neurotransmitters in rat.
Insomnia rats with heart and kidney imbalance were induced through
intraperitoneal injection with
p-chlorophenylalanine(PCPA, 400 mg·kg~(-1)·d~(-1)), and the model rats were intragastrically administrated with
Jiaotai Pills(3.3 g·kg~(-1)·d~(-1)) for 7 days. Nine
neurotransmitters were determined by UPLC-MS/MS and principal component analysis(PCA) method in serum, urine, brain, heart, liver, kidney and adrenal gland of rats. The results showed that the ratio of
5-HT and
5-HIAA in platelet of
insomnia rats was significantly lower than that in the normal group, and
Jiaotai Pills had a significant up-regulatory or down-regulatory effect. Compared with the normal group, the changed
neurotransmitters in blood of
insomnia rats were
5-HIAA, E, NE, DA, Glu and ACH, and except ACH, the changes of 7 kinds of
neurotransmitters in urine were more significant,
Jiaotai Pills had a significant up-regulatory or down-regulatory effect. Compared with the normal group, all of the 8
neurotransmitters in
insomnia rats except HVA were changed.
Jiaotai Pills could regulate the
neurotransmitters in each tissue of
insomnia rats, especially in brain, liver and adrenal gland. In conclusion,
insomnia is caused by not only a change of
neurotransmitters in brain, but also a series of changes in peripheral tissues. It indicates that
insomnia is a systematic imbalance of
neurotransmitters.
Jiaotai Pills not only regulates the central nervous system, but also has a certain protective effect on other organs, reflecting the multi-target and systematic effect of
Jiaotai Pills in the treatment of
insomnia.