The mannose 6-phosphate receptor targeted with porphyrin-based periodic mesoporous organosilica nanoparticles for rhabdomyosarcoma theranostics.

Abstract

Porphyrin-based periodic mesoporous organosilica nanoparticles (PMO) synthesized from a large functional octatriethoxysilylated porphyrin precursor and allowing two-photon excitation photodynamic therapy (TPE-PDT) and NIR imaging were synthesized. These PMO were grafted with polyethylene glycol (PEG) moieties and an analogue of mannose 6-phosphate functionalized at the anomeric position (AMFA). AMFAs are known to efficiently target mannose 6-phosphate receptors (M6PRs) which are over-expressed in various cancers. Here, we demonstrated for the first time that M6PRs were over-expressed in rhabdomyosarcoma (RMS) cells and could be efficiently targeted with PMO-AMFA allowing TPE imaging and TPE-PDT of RMS cells. The comparison with healthy myoblasts demonstrated an absence of biological effects, suggesting a cancer cell specificity in the biomedical action observed.

• Authors: Morgane Daurat , Christophe Nguyen , Sofia Dominguez Gil , Vincent Sol , Vincent Chaleix , Clarence Charnay , Laurence Raehm , Khaled El Cheikh , Alain Morère , Michele Bernasconi , Andrea Timpanaro , Marcel Garcia , Frédérique Cunin , Jochen Roessler , Jean-Olivier Durand , Magali Gary-Bobo
• Journal: Biomaterials science (Biomater Sci) Vol. 8 Issue 13 Pg. 3678-3684 (Jul 07 2020) ISSN: 2047-4849 [Electronic] England
• PMID: 32469353 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Biocompatible Materials
• Organosilicon Compounds
• Porphyrins
• Receptor, IGF Type 2

Topics

• Antineoplastic Agents (chemical synthesis, chemistry, pharmacology)
• Biocompatible Materials (chemical synthesis, chemistry, pharmacology)
• Humans
• Nanoparticles (chemistry)
• Optical Imaging
• Organosilicon Compounds (chemical synthesis, chemistry, pharmacology)
• Particle Size
• Photochemotherapy
• Porosity
• Porphyrins (chemistry, pharmacology)
• Proteomics
• Receptor, IGF Type 2 (antagonists & inhibitors, genetics)
• Rhabdomyosarcoma (diagnostic imaging, drug therapy, genetics)
• Surface Properties
• Theranostic Nanomedicine
• Tumor Cells, Cultured