Acetate promotes SNAI1 expression by ACSS2-mediated histone acetylation under glucose limitation in renal cell carcinoma cell.

Abstract

Metastasis is the main cause of cancer-associated deaths, yet this complex process is still not well understood. Many studies have shown that acetate is involved in cancer metastasis, but the molecular mechanisms remain to be elucidated. In the present study, we first measured the effect of acetate on zinc finger transcriptional repressor SNAI1 and acetyl-CoA synthetase 2 (ACSS2) under glucose limitation in renal cell carcinoma cell lines, 786-O and ACHN. Then, RNA interference and overexpression of ACSS2 were used to detect the role of acetate on SNAI1 expression and cell migration. Finally, chromatin immunoprecipitation assay (ChIP) was used to investigate the regulatory mechanism of acetate on SNAI1 expression. The results showed that acetate increased the expressions of SNAI1 and ACSS2 under glucose limitation. ACSS2 knockdown significantly decreased acetate-induced SNAI1 expression and cell migration, whereas overexpression of ACSS2 increased SNAI1 level and histone H3K27 acetylation (H3K27ac). ChIP results revealed that acetate increased H3K27ac levels in regulatory region of SNAI1, but did not increase ACSS2-binding ability. Our study identified a novel inducer, acetate, which can promote SNAI1 expression by ACSS2-mediated histone acetylation in partly. This finding has important implication in treatment of metastatic cancers.

• Authors: Lv Yao, Linying Jiang, Fuxing Zhang, Minghua Li, Bo Yang, Fangting Zhang, Xiaoqiang Guo
• Journal: Bioscience reports (Biosci Rep) Vol. 40 Issue 6 (06 26 2020) ISSN: 1573-4935 [Electronic] England
• PMID: 32458971 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 The Author(s).

Chemical References

• Acetates
• Antineoplastic Agents
• Histones
• SNAI1 protein, human
• Snail Family Transcription Factors
• ACSS2 protein, human
• Acetate-CoA Ligase
• Glucose

Topics

• Acetate-CoA Ligase (genetics, metabolism)
• Acetates (metabolism, toxicity)
• Acetylation
• Antineoplastic Agents (metabolism, toxicity)
• Carcinoma, Renal Cell (enzymology, genetics, secondary)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Gene Expression Regulation, Neoplastic
• Glucose (deficiency)
• Histones (metabolism)
• Humans
• Kidney Neoplasms (enzymology, genetics, pathology)
• Neoplasm Invasiveness
• Protein Processing, Post-Translational (drug effects)
• Signal Transduction
• Snail Family Transcription Factors (genetics, metabolism)