Dust mite-derived Enterobacterial fimbriae H protein enforces the allergen specific immunotherapy in asthma mice.

Abstract

BACKGROUND:

The mite alimentary canal contains plenty of microbiota. It is accepted that some of the microbial products function as adjuvants to speed up immune responses.

OBJECTIVES:

We identified five bacterial proteins from dust mite, and Enterobacterial fimbriae H (FimH) was one of them. This study aims to test a hypothesis that the FimH protein enforces immunotherapy in asthmatic mice.

METHODS:

Asthmatic mice were treated by allergen specific immunotherapy (ASIT) with rDer f1/f2 or rDer f1/f2 plus FimH. Changes in inflammatory cell infiltration, airway hyperreactivity, frequency of Tregs, splenic CD4+IFN-γ+ cells, and serum levels of TGF-β, IL-10, IL-13 and IL-17A of asthmatic mice were checked.

RESULTS:

ASIT with rDer f1/f2 plus FimH reduced inflammatory cell infiltration, airway hyperreactivity (AHR), and levels of IgE and IgG1 compared to ASIT with rDer f1/f2 alone, but the levels of IgG2a increased. Asthmatic mice that underwent ASIT with rDer f1/f2 plus FimH showed increased frequency of Tregs, splenic CD4+IFN-γ+ cells, serum levels of TGF-β and IL-10; and deceased splenic CD4+IL-4+ cells, and serum levels of IL-13 and IL-17A. In vitro study showed FimH triggered IL-10 expression in a concentration dependent manner and facilitated the differentiation of Tregs.

CONCLUSION:

Used as an adjuvant, FimH enforces the effect of ASIT in asthmatic mice via augmenting Tregs.

• Authors: X Yang, H Wang, D Zhao, J Wang, X Liu, X Yuan, M Zhang, G Li, P Ran, P Yang, Z Liu
• Journal: Allergologia et immunopathologia (Allergol Immunopathol (Madr)) 2020 Nov - Dec Vol. 48 Issue 6 Pg. 654-665 ISSN: 1578-1267 [Electronic] Singapore
• PMID: 32446781 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 SEICAP. Published by Elsevier España, S.L.U. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Allergens
• Antigens, Bacterial
• Antigens, Dermatophagoides
• Bacterial Proteins
• DNA-Binding Proteins
• Recombinant Proteins
• Vaccines, Synthetic
• histone-like protein HU, bacteria

Topics

• Adjuvants, Immunologic (administration & dosage)
• Allergens (administration & dosage)
• Animals
• Antigens, Bacterial (administration & dosage)
• Antigens, Dermatophagoides (administration & dosage, immunology)
• Asthma (immunology, therapy)
• Bacterial Proteins (administration & dosage)
• Cells, Cultured
• DNA-Binding Proteins (administration & dosage)
• Dendritic Cells
• Desensitization, Immunologic (methods)
• Disease Models, Animal
• Enterobacteriaceae (immunology)
• Female
• Humans
• Immunogenicity, Vaccine
• Mice
• Microbiota (immunology)
• Primary Cell Culture
• Pyroglyphidae (immunology, microbiology)
• Recombinant Proteins (administration & dosage, immunology)
• Vaccines, Synthetic (administration & dosage, immunology)