Abstract | INTRODUCTION: METHODS: In this study, BDMC was injected into the lateral ventricles of normal C57BL/6 mice, APP/PS mice, and APP/PS mice treated with EX527 (the inhibitor of SIRT1). Y maze and Morris water maze were used to test the learning and memory ability of mice. Nissl staining was used to observe the morphological changes of neurons. Immunofluorescence staining was used to detect Aβ deposition in mice. The activities of GSH and SOD were determined to observe the levels of oxidative stress in mice. And Western blot analyses were used to detect content of SIRT1 in mice. RESULTS: In the APP/PS mice, after BDMC intervention, their cognitive function improved, oxidative stress adjusted, the number of neurons increased, Aβ deposition decreased, and the level of SIRT1 expression increased. However, when SIRT1 is inhibited, BDMC on the improvement in the learning and memory ability and the improvement on oxidative stress in APP/PS1 mice were reversed. CONCLUSION: Our findings demonstrated that in the AD mice, BDMC has antagonistic effect on AD. And an intermediate step in the antagonism effect is caused by SIRT1 upregulation, which leading to decreased oxidative stress. Based on these, we concluded that BDMC injection into the lateral ventricle can act against AD by upregulating SIRT1 to antioxidative stress.
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Authors | Yan Xu, Rong Hu, Duanqun He, Guijuan Zhou, Heng Wu, Chenlin Xu, Bing He, Lin Wu, Yilin Wang, Yunqian Chang, Rundong Ma, Ming Xie, Zijian Xiao |
Journal | Brain and behavior
(Brain Behav)
Vol. 10
Issue 7
Pg. e01655
(07 2020)
ISSN: 2162-3279 [Electronic] United States |
PMID | 32441492
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2020 The Authors. Brain and Behavior published by Wiley Periodicals LLC. |
Chemical References |
- Diarylheptanoids
- bisdemethoxycurcumin
- Sirt1 protein, mouse
- Sirtuin 1
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Topics |
- Alzheimer Disease
(drug therapy, metabolism)
- Animals
- Diarylheptanoids
(pharmacology)
- Disease Models, Animal
- Female
- Male
- Maze Learning
(drug effects)
- Memory
(drug effects)
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Oxidative Stress
(drug effects)
- Sirtuin 1
(metabolism)
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