Evaluation of Efficacy of Injectable-guided Tissue Regeneration with and without Clindamycin on the Colonization of Porphyromonas gingivalis by Real-time Polymerase Chain Reaction.

Abstract

AIM:

Aim of the present study was to evaluate the loading of clindamycin with injectable-guided tissue regeneration (GTR) will prevent the colonization of Porphyromonas gingivalis and to compare and assess the quantitative changes in P. gingivalis colony forming units (CFUs) by real-time polymerase chain reaction (PCR) analysis.

MATERIALS AND METHODS:

Thirty microbiological samples were pooled from the deepest periodontal pockets from the thirty sites from the two groups: group I-injectable GTR placed in the defect filled with demineralized freeze-dried bone allograft (DFDBA) and group II-clindamycin loaded injectable GTR placed in the defect filled with DFDBA. The total number of P. gingivalis CFUs was estimated using real-time PCR at baseline and 4 weeks after therapy.

RESULTS:

A significant reduction in P. gingivalis CFUs at the end of 4 weeks was seen in both groups. Comparative evaluations between both groups at 4 weeks were with a mean of 4.44 ± 2.28 and 4.75 ± 3.32, respectively. Though there was a significant reduction in group II, the difference was statistically insignificant.

CONCLUSION:

The results suggest that clindamycin is beneficial in reducing microbial infection and can potentiate regeneration through host modulation.

CLINICAL SIGNIFICANCE:

Injectable GTR has the ability to mold according to the defect size and shape and eliminates the need to manipulate the membrane as required for the conventional membrane.

• Authors: Voruganti Deepak, Johnson J Josephin, Palaparthy R Babu, Vikram R Guntakandla, Jagadish R Gooty, Suryakanth Malgikar, Abhinav Gupta
• Journal: The journal of contemporary dental practice (J Contemp Dent Pract) Vol. 21 Issue 1 Pg. 36-40 (Jan 01 2020) ISSN: 1526-3711 [Electronic] India
• PMID: 32381798 (Publication Type: Journal Article)

Chemical References

• Clindamycin

Topics

• Bone Transplantation
• Clindamycin
• Guided Tissue Regeneration, Periodontal
• Porphyromonas gingivalis
• Real-Time Polymerase Chain Reaction