Abstract | INTRODUCTION: MATERIAL AND METHODS:
Sepsis was induced by cecal ligation and puncture (CLP); rats were treated with 30 or 100 mg/kg of myricitrin for 5 days prior to the induction of CLP. The effect of myricitrin was observed by determining the neurological function using the open field test and step-down inhibitory avoidance test. Cerebral oedema and the levels of inflammatory and oxidative stress mediators were determined in brain tissues. Moreover, the expression levels of Bcl-2, Bax, IκB-α, nuclear factor κB (NF-κB), caspase-3 and NLRP3 were estimated in brain tissues by Western blotting and the mRNA expression of NF-κB, caspase-3 and NLRP3 in brain tissues was estimated by real-time polymerase chain reaction. An immunofluorescence assay was performed to estimate inflammasome activity. RESULTS: The results suggest that treatment with myricitrin protects neuronal function in rats with CLP-induced sepsis. Decreases in inflammation and oxidative stress mediators were observed in the brain tissues of the myricitrin-treated group compared to the CLP group. Moreover, treatment with myricitrin ameliorated the altered Bcl-2, Bax, IκB-α, NF-κB, caspase-3 and NLRP3 protein and mRNA expression levels in the brain tissues of septic rats. CONCLUSIONS: The data reveal that myricitrin ameliorated neuroinflammation and improved memory in rats with CLP-induced sepsis by regulating the NLRP3/Bax/Bcl signalling pathway.
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Authors | Junzuo Gong, Shu Luo, Shiqiao Zhao, Songlin Yin, Xiaofeng Li, Tianyi Mou |
Journal | Folia neuropathologica
(Folia Neuropathol)
Vol. 57
Issue 4
Pg. 327-334
( 2019)
ISSN: 1509-572X [Electronic] Poland |
PMID | 32337945
(Publication Type: Journal Article)
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Chemical References |
- Flavonoids
- NF-kappa B
- NLR Family, Pyrin Domain-Containing 3 Protein
- Nlrp3 protein, rat
- myricitrin
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Topics |
- Animals
- Flavonoids
(pharmacology)
- Inflammation
(drug therapy)
- Male
- Memory
(drug effects)
- NF-kappa B
(drug effects, metabolism)
- NLR Family, Pyrin Domain-Containing 3 Protein
(drug effects)
- Oxidative Stress
(drug effects)
- Rats, Wistar
- Sepsis
(drug therapy)
- Sepsis-Associated Encephalopathy
(drug therapy)
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