Prx2 (Peroxiredoxin 2) as a Cause of Hydrocephalus After Intraventricular Hemorrhage.

Abstract

Background and Purpose- Our recent study demonstrated that release of Prx2 (peroxiredoxin 2) from red blood cells (RBCs) is involved in the inflammatory response and brain injury after intracerebral hemorrhage. The current study investigated the role of extracellular Prx2 in hydrocephalus development after experimental intraventricular hemorrhage. Methods- There were 4 parts in this study. First, Sprague-Dawley rats received an intraventricular injection of lysed RBC or saline and were euthanized at 1 hour for Prx2 measurements. Second, rats received an intraventricular injection of Prx2, deactivated Prx2, or saline. Third, lysed RBC was coinjected with conoidin A, a Prx2 inhibitor, or vehicle. Fourth, rats received Prx2 injection and were treated with minocycline or saline (i.p.). The effects of Prx2 and the inhibitors were examined using magnetic resonance imaging assessing ventriculomegaly, histology assessing ventricular wall damage, and immunohistochemistry to assess inflammation, particularly at the choroid plexus. Results- Intraventricular injection of lysed RBC resulted in increased brain Prx2 and hydrocephalus. Intraventricular injection of Prx2 alone caused hydrocephalus, ventricular wall damage, activation of choroid plexus epiplexus cells (macrophages), and an accumulation of neutrophils. Conoidin A attenuated lysed RBC-induced injury. Systemic minocycline treatment reduced the epiplexus cell activation and hydrocephalus induced by Prx2. Conclusions- Prx2 contributed to the intraventricular hemorrhage-induced hydrocephalus, probably by inducing inflammatory responses in choroid plexus and ventricular wall damage.

• Authors: Xiaoxiao Tan, Jingyin Chen, Richard F Keep, Guohua Xi, Ya Hua
• Journal: Stroke (Stroke) Vol. 51 Issue 5 Pg. 1578-1586 (05 2020) ISSN: 1524-4628 [Electronic] United States
• PMID: 32279622 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Anti-Inflammatory Agents
• Quinoxalines
• conoidin A
• Peroxiredoxins
• Prdx2 protein, rat
• Minocycline

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cerebral Intraventricular Hemorrhage (complications, metabolism)
• Choroid Plexus (drug effects, metabolism, pathology)
• Disease Models, Animal
• Ependyma (drug effects, pathology)
• Female
• Hydrocephalus (etiology, metabolism)
• Hylobatidae
• Inflammation (metabolism, pathology)
• Injections, Intraventricular
• Macrophage Activation (drug effects)
• Macrophages (drug effects, metabolism, pathology)
• Male
• Minocycline (pharmacology)
• Neutrophils (drug effects, pathology)
• Peroxiredoxins (antagonists & inhibitors, metabolism, pharmacology)
• Quinoxalines (pharmacology)
• Rats
• Rats, Sprague-Dawley