MS-IPA1 is a new synthetic compound that is synthesized from
tryptamine. Recently, our group demonstrated that SST-VED-I-1, which has a similar chemical structure to
MS-IPA1, inhibits
starvation-induced apoptosis in osteoblasts. However, the effects of
MS-IPA1 on apoptosis in osteoblasts have not yet been examined. Therefore, this study examined the effects of this compound on apoptosis in osteoblasts. In this study, MC3T3-E1 mouse osteoblasts were used and apoptosis was induced by ultraviolet radiation (UV). We investigated the effect of
MS-IPA1 on apoptosis by analyzing caspase3/7 activity, translocation of
phosphatidylserine (PS), and
mRNA expression levels of Bcl-2 and Bax. In addition, it was investigated whether
MS-IPA1 affects cell proliferation and cell cycle progression. We found that
MS-IPA1 had no effect on cell proliferation or cell cycle progression. However,
MS-IPA1 suppressed UV-induced cell death in a dose-dependent manner, which was accompanied with the inhibition of
caspase activation and translocation of PS. Furthermore, after UV exposure, Bcl-2 expression was increased in the MS-IPA1-treated cells as compared to that in the vehicle-treated cells. In contrast, Bax expression was decreased in the MS-IPA1-treated cell as compared to that in the vehicle-treated cells. These results suggest that
MS-IPA1 has an inhibitory effect on apoptosis in osteoblasts through a Bcl-2 family-dependent signaling pathway.