Inhibition of glioma proliferation and migration by magnetic nanoparticle mediated JAM-2 silencing.

Abstract

Brain invasion is a biological hallmark of glioma that leads to its aggressiveness and prognosis. Junctional adhesion molecule-2 (JAM-2) was found to be overexpressed in human glioma. In this study, the effects of JAM-2 silencing mediated by cell-penetrating magnetic nanoparticles were investigated on glioma cell proliferation and migration in vitro and in vivo. The results showed that the deregulation of JAM-2 in glioma cell lines could cause a dramatic decrease in cell proliferation and migration in vitro. The expression level of cytoskeleton remodeling and migration associated protein genes appeared to be a downstream factor of JAM-2. Furthermore, silencing of JAM-2 expression in implanted glioma cells was found to impair in vivo tumor growth significantly. These data provide new evidence for the role of JAM-2 in the progression of glioma and show its great potential in human glioma gene therapeutics.

• Authors: Lifeng Qi , Jing Liu , Haiyan Zhu , Zhuoquan Li , Kun Lu , Tian Li , Donglu Shi
• Journal: Journal of materials chemistry. B (J Mater Chem B) Vol. 2 Issue 41 Pg. 7168-7175 (Nov 07 2014) ISSN: 2050-7518 [Electronic] England
• PMID: 32261795 (Publication Type: Journal Article)