Spectrally Combined Encoding for Profiling Heterogeneous Circulating Tumor Cells Using a Multifunctional Nanosphere-Mediated Microfluidic Platform.

Abstract

Comprehensive phenotypic profiling of heterogeneous circulating tumor cells (CTCs) at single-cell resolution has great importance for cancer management. Herein, a novel spectrally combined encoding (SCE) strategy was proposed for multiplex biomarker profiling of single CTCs using a multifunctional nanosphere-mediated microfluidic platform. Different cellular biomarkers uniquely labeled by multifunctional nanosphere barcodes, possessing identical magnetic tags and distinct optical signatures, enabled isolation of heterogeneous CTCs with over 91.6 % efficiency and in situ SCE of phenotypes. By further trapping individual CTCs in ordered microstructures on chip, composite single-cell spectral signatures were conveniently and efficiently obtained, allowing reliable spectral-readout for multiplex biomarker profiling. This SCE strategy exhibited great potential in multiplex profiling of heterogeneous CTC phenotypes, offering new avenues for cancer study and precise medicine.

• Authors: Ling-Ling Wu, Zhi-Ling Zhang, Man Tang, Dong-Liang Zhu, Xiao-Juan Dong, Jiao Hu, Chu-Bo Qi, Hong-Wu Tang, Dai-Wen Pang
• Journal: Angewandte Chemie (International ed. in English) (Angew Chem Int Ed Engl) Vol. 59 Issue 28 Pg. 11240-11244 (07 06 2020) ISSN: 1521-3773 [Electronic] Germany
• PMID: 32246736 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Biomarkers, Tumor

Topics

• Biomarkers, Tumor (blood)
• Cell Line, Tumor
• DNA Barcoding, Taxonomic
• Humans
• Microfluidics
• Microscopy, Fluorescence
• Nanospheres
• Neoplastic Cells, Circulating
• Proof of Concept Study