Abstract |
Skin defects caused by various reasons are currently common clinical problems. At present, hydrogels have been proposed as tissue-engineered skin scaffolds to regenerate the tissues of the defect. We used human-like collagen (HLC), which was isolated and purified after high-density fermentation of recombinant E. coli BL21 The gel uses HLC and carboxymethylated chitosan (CCS) as raw materials and combines enzyme-chemical double cross-linking technology to form a three-dimensional porous network structure that mimics the human extracellular matrix, providing attachment points and nutrients for cell growth and proliferation. For comparison, we used a common hydrogel raw material, gelatin, to prepare a hydrogel in the same way. The experimental results show that the HLC-CCS skin scaffold hydrogel has good mechanical properties, high porosity and good histocompatibility. And full-thickness skin defect repair experiments show that this hydrogel has a good ability to promote skin tissue regeneration at the wound. In summary, this HLC-based double-crosslinked hydrogel can be used as a project strategy for skin defect repair.
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Authors | Jing Cao, Pan Wang, Yannan Liu, Chenhui Zhu, Daidi Fan |
Journal | International journal of biological macromolecules
(Int J Biol Macromol)
Vol. 155
Pg. 625-635
(Jul 15 2020)
ISSN: 1879-0003 [Electronic] Netherlands |
PMID | 32240736
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Elsevier B.V. All rights reserved. |
Chemical References |
- Biocompatible Materials
- Hydrogels
- Protective Agents
- Collagen
- Chitosan
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Topics |
- Animals
- Biocompatible Materials
(pharmacology)
- Chitosan
(analogs & derivatives, pharmacology)
- Collagen
(chemistry)
- Humans
- Hydrogels
(chemistry)
- Protective Agents
(pharmacology)
- Rabbits
- Skin
(drug effects, injuries)
- Tissue Engineering
- Tissue Scaffolds
(chemistry)
- Wound Healing
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