Abstract | BACKGROUND: AIM OF THE STUDY: METHODS: Biopsies from DFU patients grade 3 according to Wagner's classification, from diabetic patients without ulcer and from healthy donors were obtained. qPCR, immunohistochemistry and cell line cultures were performed. To assess whether L-isoleucine, calcitriol, phenyl butyrate, metformin, glyburide or insulin induced RNase 7, keratinocytes were stimulated, and RNase 7 expression was evaluated. RESULTS: Our data showed that RNase 7 levels were decreased in both diabetic groups when were compared with skin from healthy donors. Since most of the DM2 patients are treated with drugs to reduce glycemia, we investigated whether glyburide, metformin or insulin were able to induce any change regarding RNase 7 production. Results showed that metformin reduces the expression of RNase 7 in in vitro treated keratinocytes, suggesting that the chronic use of metformin should be evaluated in DFU patients, whereas calcitriol, phenyl butyrate and L-isoleucine did not increase the RNase 7 production. CONCLUSIONS: Due RNase 7 has antimicrobial activity, its downregulation can make prone to DM2 patients to develop infections and impaired wound healing.
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Authors | Adrian Rodríguez-Carlos, Valentin Trujillo, Irma Gonzalez-Curiel, Sara Marin-Luevano, Flor Torres-Juarez, Alan Santos-Mena, Cesar Rivas-Santiago, Jose A Enciso-Moreno, Veronica Zaga-Clavellina, Bruno Rivas-Santiago |
Journal | Archives of medical research
(Arch Med Res)
Vol. 51
Issue 4
Pg. 327-335
(05 2020)
ISSN: 1873-5487 [Electronic] United States |
PMID | 32229156
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 IMSS. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Metformin
- Ribonucleases
- Ribonuclease 7
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Topics |
- Adult
- Chronic Disease
- Diabetes Mellitus, Type 2
(genetics)
- Diabetic Foot
(genetics)
- Down-Regulation
- Female
- Humans
- Male
- Metformin
(adverse effects)
- Middle Aged
- Ribonucleases
(metabolism)
- Wound Healing
(drug effects)
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