Effects of Paeonia lactiflora Extract on Estrogen Receptor β, TPH2, and SERT in Rats with PMS Anxiety.

Abstract

This study is to investigate the effect of Paeonia lactiflora extract on PMS anxiety and on expression of estrogen receptor β (ERβ), tryptophan hydroxylase-2 (TPH2), and serotonin transporter (SERT) in the premenstrual syndrome (PMS) anxiety model rats. The vaginal smear and open field test were used to screen rats in nonreception phase of estrus cycle with similar macroscopic behaviors and regular estrus cycle. PMS anxiety model rats were prepared by electrical stimulation. RT-PCR and immunofluorescence were used to measure the expression of ERβ, TPH2, and SERT. Compared with normal rats, the total distance in the open field test of the model rats was significantly increased (P < 0.05). The model rats showed nervous alertness, irritability, and sensitivity to external stimuli. After treatment with the Paeonia lactiflora extract, the total distance of rats was significantly reduced (P < 0.05). In reception stage, there was no significant difference in the mRNA and protein expression of ERβ, TPH2, and SERT. In nonreception stage, the expression of ERβ and TPH2 in the model group was significantly decreased (P < 0.05) as compared with the control group, but not SERT. Abnormal changes of the above indicators were reversed after the administration of the Paeonia lactiflora extract. In conclusion, Paeonia lactiflora extract can increase the expression of ERβ and TPH2 and decrease SERT in PMS model rats, which may be one of the mechanisms underlying the effect of Paeonia lactiflora extract on PMS.

• Authors: Jieqiong Wang, Chunhong Song, Dongmei Gao, Sheng Wei, Wenjun Sun, Yinghui Guo, Shiguang Sun, Xi Tian, Huihao Li, Mingqi Qiao
• Journal: BioMed research international (Biomed Res Int) Vol. 2020 Pg. 4690504 ( 2020) ISSN: 2314-6141 [Electronic] United States
• PMID: 32219134 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Jieqiong Wang et al.

Chemical References

• Estrogen Receptor beta
• Plant Extracts
• Serotonin Plasma Membrane Transport Proteins
• Tryptophan Hydroxylase
• tph2 protein, rat

Topics

• Animals
• Anxiety (complications, metabolism)
• Anxiety Disorders (complications, metabolism)
• Disease Models, Animal
• Estrogen Receptor beta (drug effects, metabolism)
• Female
• Male
• Paeonia (chemistry)
• Plant Extracts (pharmacology)
• Premenstrual Syndrome (complications)
• Rats
• Rats, Wistar
• Serotonin Plasma Membrane Transport Proteins (drug effects, metabolism)
• Tryptophan Hydroxylase (drug effects, metabolism)