Antibacterial activity of salvia officinalis L. against periodontopathogens: An in vitro study.

Abstract

Being aware of the remarkable antimicrobial potential of S. officinalis L., we aimed to evaluate the antimicrobial activity of the S. officinalis dichloromethane crude extract (SOD), dichloromethane-soluble fractions (SODH and SODD), SODD subfractions (SODD1 and SODD2), and pure substances (manool, salvigenin, and viridiflorol) against periodontopathogens. This bioassay-guided study comprises five antimicrobial tests-determination of the Minimum Inhibitory Concentration (MIC), determination of the Minimum Bactericidal Concentration (MBC), determination of the antibiofilm activity, construction of the Time-kill curve (determination of Bactericidal Kinetics), and determination of the Fractional Inhibitory Concentration Index-on six clinical bacterial isolates and three standard bacterial strains involved in periodontal disease. SOD has moderate activity against most of the tested bacteria, whereas SODD1, SODH1, SODH3, and manool afford the lowest results. The Porphyromonas gingivalis (ATTC and clinical isolate) biofilm is considerably resistant to all the samples. In association with chlorhexidine gluconate, only SODH1 exerts additive action against P. gingivalis (clinical isolate). Therefore, SODH1 and manool are promising antibacterial agents and may provide therapeutic solutions for periodontal infections.

• Authors: Filipe Santos Ferreira Mendes, Letícia Matanovich Garcia, Thais da Silva Moraes, Luciana Assirati Casemiro, Clauber Barbosa de Alcântara, Sérgio Ricardo Ambrósio, Rodrigo Cássio Sola Veneziani, Mayker Lazaro Dantas Miranda, Carlos Henrique Gomes Martins
• Journal: Anaerobe (Anaerobe) Vol. 63 Pg. 102194 (Jun 2020) ISSN: 1095-8274 [Electronic] England
• PMID: 32205191 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Diterpenes
• Plant Extracts
• manool

Topics

• Aggressive Periodontitis (drug therapy, microbiology)
• Anti-Bacterial Agents (pharmacology)
• Bacteria (drug effects)
• Biofilms (drug effects)
• Diterpenes (pharmacology)
• Humans
• Microbial Sensitivity Tests
• Mouth (microbiology)
• Plant Extracts (pharmacology)
• Porphyromonas gingivalis (drug effects)
• Salvia officinalis (metabolism)