Plant growth regulators and Axl and immune checkpoint inhibitors from the edible mushroom Leucopaxillus giganteus.

Abstract

A novel compound, (R)-4-ethoxy-2-hydroxy-4-oxobutanoic acid (1), and six known compounds (2-7) were isolated from the fruiting bodies of the wild edible mushroom Leucopaxillus giganteus. The planar structure of 1 was determined by the interpretation of spectroscopic data analysis. The absolute configuration of 1 was determined by comparing specific rotation of the synthetic compounds. In the plant regulatory assay, the isolated compounds (1-7) and the chemically prepared compounds (8-10) were evaluated their biological activity against the lettuce (Lactuca sativa) growth. Compounds 1 and 3-10 showed the significant regulatory activity of lettuce growth. 1 showed the strongest inhibition activity among the all the compounds tested. In the lung cancer assay, all the compounds were assessed the mRNA expression of Axl and immune checkpoints (PD-L1, PD-L2) in the human A549 alveolar epithelial cell line by RT-PCR. Compounds 1-10 showed significant inhibition activity against Axl and/or immune checkpoint.

• Authors: Irine Yunhafita Malya, Jing Wu, Etsuko Harada, Masaaki Toda, Corina N D'Alessandro-Gabazza, Taro Yasuma, Esteban C Gabazza, Jae-Hoon Choi, Hirofumi Hirai, Hirokazu Kawagishi
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 84 Issue 7 Pg. 1332-1338 (Jul 2020) ISSN: 1347-6947 [Electronic] England
• PMID: 32200702 (Publication Type: Journal Article)

Chemical References

• B7-H1 Antigen
• CD274 protein, human
• Immune Checkpoint Inhibitors
• PDCD1LG2 protein, human
• Plant Growth Regulators
• Programmed Cell Death 1 Ligand 2 Protein
• Proto-Oncogene Proteins
• Receptor Protein-Tyrosine Kinases
• Axl Receptor Tyrosine Kinase

Topics

• A549 Cells
• Adenocarcinoma, Bronchiolo-Alveolar (metabolism, pathology)
• Agaricales (chemistry)
• B7-H1 Antigen (antagonists & inhibitors, metabolism)
• Fruiting Bodies, Fungal (chemistry)
• Humans
• Immune Checkpoint Inhibitors (pharmacology)
• Lactuca (drug effects, growth & development)
• Lung Neoplasms (metabolism, pathology)
• Plant Growth Regulators (pharmacology)
• Programmed Cell Death 1 Ligand 2 Protein (antagonists & inhibitors, metabolism)
• Proto-Oncogene Proteins (antagonists & inhibitors, metabolism)
• Receptor Protein-Tyrosine Kinases (antagonists & inhibitors, metabolism)
• Signal Transduction (drug effects)
• Axl Receptor Tyrosine Kinase