Polyphenolic Compounds from Lespedeza Bicolor Root Bark Inhibit Progression of Human Prostate Cancer Cells via Induction of Apoptosis and Cell Cycle Arrest.

Abstract

From a root bark of Lespedeza bicolor Turch we isolated two new (7 and 8) and six previously known compounds (1-6) belonging to the group of prenylated polyphenols. Their structures were elucidated using mass spectrometry, nuclear magnetic resonance and circular dichroism spectroscopy. These natural compounds selectively inhibited human drug-resistant prostate cancer in vitro. Prenylated pterocarpans 1-3 prevented the cell cycle progression of human cancer cells in S-phase. This was accompanied by a reduced expression of mRNA corresponding to several human cyclin-dependent kinases (CDKs). In contrast, compounds 4-8 induced a G1-phase cell cycle arrest without any pronounced effect on CDKs mRNA expression. Interestingly, a non-substituted hydroxy group at C-8 of ring D of the pterocarpan skeleton of compounds 1-3 seems to be important for the CDKs inhibitory activity.

• Authors: Sergey A Dyshlovoy, Darya Tarbeeva, Sergey Fedoreyev, Tobias Busenbender, Moritz Kaune, Marina Veselova, Anatoliy Kalinovskiy, Jessica Hauschild, Valeria Grigorchuk, Natalya Kim, Carsten Bokemeyer, Markus Graefen, Petr Gorovoy, Gunhild von Amsberg
• Journal: Biomolecules (Biomolecules) Vol. 10 Issue 3 (03 14 2020) ISSN: 2218-273X [Electronic] Switzerland
• PMID: 32183314 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Polyphenols

Topics

• Apoptosis (drug effects)
• Cell Cycle Checkpoints (drug effects)
• Humans
• Lespedeza (chemistry)
• Male
• PC-3 Cells
• Plant Bark (chemistry)
• Plant Roots (chemistry)
• Polyphenols (chemistry, pharmacology)
• Prostatic Neoplasms (drug therapy, metabolism, pathology)