Loss of IKK Subunits Limits NF-κB Signaling in Reovirus-Infected Cells.

Abstract

Viruses commonly antagonize innate immune pathways that are primarily driven by nuclear factor kappa B (NF-κB), interferon regulatory factor (IRF), and the signal transducer and activator of transcription proteins (STAT) family of transcription factors. Such a strategy allows viruses to evade immune surveillance and maximize their replication. Using an unbiased transcriptome sequencing (RNA-seq)-based approach to measure gene expression induced by transfected viral genomic RNA (vgRNA) and reovirus infection, we discovered that mammalian reovirus inhibits host cell innate immune signaling. We found that, while vgRNA and reovirus infection both induce a similar IRF-dependent gene expression program, gene expression driven by the NF-κB family of transcription factors is lower in infected cells. Potent agonists of NF-κB such as tumor necrosis factor alpha (TNF-α) and vgRNA failed to induce NF-κB-dependent gene expression in infected cells. We demonstrate that NF-κB signaling is blocked due to loss of critical members of the inhibitor of kappa B kinase (IKK) complex, NF-κB essential modifier (NEMO), and IKKβ. The loss of the IKK complex components prevents nuclear translocation and phosphorylation of NF-κB, thereby preventing gene expression. Our study demonstrates that reovirus infection selectively blocks NF-κB, likely to counteract its antiviral effects and promote efficient viral replication.IMPORTANCE Host cells mount a response to curb virus replication in infected cells and prevent spread of virus to neighboring, as yet uninfected, cells. The NF-κB family of proteins is important for the cell to mediate this response. In this study, we show that in cells infected with mammalian reovirus, NF-κB is inactive. Further, we demonstrate that NF-κB is rendered inactive because virus infection results in reduced levels of upstream intermediaries (called IKKs) that are needed for NF-κB function. Based on previous evidence that active NF-κB limits reovirus infection, we conclude that inactivating NF-κB is a viral strategy to produce a cellular environment that is favorable for virus replication.

• Authors: Andrew J McNamara, Pranav Danthi
• Journal: Journal of virology (J Virol) Vol. 94 Issue 10 (05 04 2020) ISSN: 1098-5514 [Electronic] United States
• PMID: 32161168 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2020 American Society for Microbiology.

Chemical References

• NF-kappa B
• Tumor Necrosis Factor-alpha
• I-kappa B Kinase

Topics

• Animals
• Cell Line
• Gene Expression Regulation
• I-kappa B Kinase (genetics, metabolism, pharmacology)
• Mice
• Mice, Knockout
• NF-kappa B (genetics, metabolism)
• Reoviridae (drug effects, genetics, physiology)
• Reoviridae Infections (metabolism)
• Signal Transduction (drug effects)
• Transcriptome
• Tumor Necrosis Factor-alpha (metabolism)