Abstract | OBJECTIVE: MATERIALS AND METHODS: The effects of AKBA on the cell viability in A549, H460, H1299, and BEAS-2B cells were determined by the CCK-8 assay. The colony formation assay was used to identify the effects of AKBA on cell proliferation. Potential roles of AKBA in regulating the cell cycle, apoptosis, and autophagy in A549 were evaluated by flow cytometry, Western blotting, reverse transcription-polymerase chain reaction (PCR) and immunofluorescence (IF). RESULTS: AKBA reduced cell viability in A549, H460, H1299, and BEAS-2B. In A549 cells, AKBA suppressed the clone formation, arrested the cell cycle at the G0/G1 phase, induced cellular apoptosis. We found that AKBA suppressed the formation of autolysosome, and decreased the expression levels of Beclin-1, LC3A/B-I, and LC3A/B-II proteins. Furthermore, AKBA also inhibited the expression levels of PI3K/Akt signaling pathway proteins. CONCLUSION: AKBA exerts the anti- cancer effects via cell cycle arrest, apoptosis induction, and autophagy suppression in NSCLC cells. This body of evidence supports the potential of AKBA as a promising drug in the treatment of NSCLC.
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Authors | Minghe Lv, Shali Shao, Qi Zhang, Xibing Zhuang, Tiankui Qiao |
Journal | OncoTargets and therapy
(Onco Targets Ther)
Vol. 13
Pg. 733-744
( 2020)
ISSN: 1178-6930 [Print] New Zealand |
PMID | 32158225
(Publication Type: Journal Article)
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Copyright | © 2020 Lv et al. |