System-Wide Analysis of Protein Acetylation and Ubiquitination Reveals a Diversified Regulation in Human Cancer Cells.

Abstract

Post-translational modifications are known to be widely involved in the regulation of various biological processes, through the extensive diversification of each protein function at the cellular network level. In order to unveil the system-wide function of the protein lysine modification in cancer cell signaling, we performed global acetylation and ubiquitination proteome analyses of human cancer cells, based on high-resolution nanoflow liquid chromatography-tandem mass spectrometry, in combination with the efficient biochemical enrichment of target modified peptides. Our large-scale proteomic analysis enabled us to identify more than 5000 kinds of ubiquitinated sites and 1600 kinds of acetylated sites, from representative human cancer cell lines, leading to the identification of approximately 900 novel lysine modification sites in total. Very interestingly, 236 lysine residues derived from 141 proteins were found to be modified with both ubiquitination and acetylation. As a consequence of the subsequent motif extraction analyses, glutamic acid (E) was found to be highly enriched at the position (-1) for the lysine acetylation sites, whereas the same amino acid was relatively dispersed along the neighboring residues of the lysine ubiquitination sites. Our pathway analysis also indicated that the protein translational control pathways, such as the eukaryotic initiation factor 2 (EIF2) and the ubiquitin signaling pathways, were highly enriched in both of the acetylation and ubiquitination proteome data at the network level. This report provides the first integrative description of the protein acetylation and ubiquitination-oriented systematic regulation in human cancer cells.

• Authors: Hiroko Kozuka-Hata, Aya Kitamura, Tomoko Hiroki, Aiko Aizawa, Kouhei Tsumoto, Jun-Ichiro Inoue, Masaaki Oyama
• Journal: Biomolecules (Biomolecules) Vol. 10 Issue 3 (03 06 2020) ISSN: 2218-273X [Electronic] Switzerland
• PMID: 32155916 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Neoplasm Proteins
• Proteome

Topics

• Acetylation
• HeLa Cells
• Humans
• MCF-7 Cells
• Neoplasm Proteins (genetics, metabolism)
• Neoplasms (genetics, metabolism, pathology)
• Proteome (genetics, metabolism)
• Proteomics
• Ubiquitination