Antibacterial Monoclonal Antibodies Do Not Disrupt the Intestinal Microbiome or Its Function.

Abstract

Antibiotics revolutionized the treatment of infectious diseases; however, it is now clear that broad-spectrum antibiotics alter the composition and function of the host's microbiome. The microbiome plays a key role in human health, and its perturbation is increasingly recognized as contributing to many human diseases. Widespread broad-spectrum antibiotic use has also resulted in the emergence of multidrug-resistant pathogens, spurring the development of pathogen-specific strategies such as monoclonal antibodies (MAbs) to combat bacterial infection. Not only are pathogen-specific approaches not expected to induce resistance in nontargeted bacteria, but they are hypothesized to have minimal impact on the gut microbiome. Here, we compare the effects of antibiotics, pathogen-specific MAbs, and their controls (saline or control IgG [c-IgG]) on the gut microbiome of 7-week-old, female, C57BL/6 mice. The magnitude of change in taxonomic abundance, bacterial diversity, and bacterial metabolites, including short-chain fatty acids (SCFA) and bile acids in the fecal pellets from mice treated with pathogen-specific MAbs, was no different from that with animals treated with saline or an IgG control. Conversely, dramatic changes were observed in the relative abundance, as well as alpha and beta diversity, of the fecal microbiome and bacterial metabolites in the feces of all antibiotic-treated mice. Taken together, these results indicate that pathogen-specific MAbs do not alter the fecal microbiome like broad-spectrum antibiotics and may represent a safer, more-targeted approach to antibacterial therapy.

• Authors: Omari Jones-Nelson, Andrey Tovchigrechko, Matthew S Glover, Fiona Fernandes, Udaya Rangaswamy, Hui Liu, David E Tabor, Jonathan Boyd, Paul Warrener, Jose Martinez, Jamese J Hilliard, C Ken Stover, Wen Yu, Gina DAngelo, Sonja Hess, Taylor S Cohen, Bret R Sellman
• Journal: Antimicrobial agents and chemotherapy (Antimicrob Agents Chemother) Vol. 64 Issue 5 (04 21 2020) ISSN: 1098-6596 [Electronic] United States
• PMID: 32152087 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Jones-Nelson et al.

Chemical References

• Anti-Bacterial Agents
• Antibodies, Monoclonal
• Bile Acids and Salts
• DNA, Bacterial
• Fatty Acids
• RNA, Ribosomal, 16S

Topics

• Animals
• Anti-Bacterial Agents (pharmacology)
• Antibodies, Monoclonal (pharmacology)
• Bile Acids and Salts (metabolism)
• DNA, Bacterial (analysis)
• Fatty Acids (metabolism)
• Feces (microbiology)
• Female
• Gastrointestinal Microbiome (drug effects)
• Mice
• Mice, Inbred C57BL
• RNA, Ribosomal, 16S (genetics)
• Specific Pathogen-Free Organisms