Abstract |
A procedure is described to measure curcumin (C), demethoxycurcumin (DMC), bisdemethoxycurcumin (BDMC), tetrahydrocurcumim (TC) and their glucuronidated metabolites (CG, DMCG, and BDMCG) in plasma, brain, liver and tumor samples. The procedure involves converting the analytes to their boron difluoride derivatives and analyzing them by combined liquid chromatography coupled to an ion trap mass spectrometer operating in the negative ion MSn scan mode. The method has superb limits of detection of 0.01 nM for all curcuminoids and 0.5 nM for TC and the glucuroniated metabolites, and several representative chromatograms of biological samples containing these analytes are provided. In addition, the pharmacokinetic profile of these compounds in one human who daily consumed an over-the-counter curcuminoid product shows the peak and changes in circulating concentrations achieved by this mode of administration.
|
Authors | Alexander J Yoon, Haiqiang Wu, Roy D Pan, Bruce Teter, Jack Cipolla, Edwin Chang, Luis Z Avila, Saroj K Basak, Eri S Srivatsan, Marilene B Wang, Greg M Cole, Sally A Frautschy, Phillip D Hampton, Kym F Faull |
Journal | Analytical biochemistry
(Anal Biochem)
Vol. 596
Pg. 113636
(05 01 2020)
ISSN: 1096-0309 [Electronic] United States |
PMID | 32081619
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
|
Copyright | Copyright © 2020. Published by Elsevier Inc. |
Chemical References |
- Boranes
- Diarylheptanoids
- boron trifluoride
|
Topics |
- Animals
- Boranes
(chemistry)
- Chromatography, Liquid
- Diarylheptanoids
(blood, chemistry, isolation & purification)
- Healthy Volunteers
- Humans
- Mass Spectrometry
- Mice
- Molecular Structure
|