Abstract |
Circular RNAs ( circRNAs) have been identified in diverse cancers for their role in regulating multiple cellular processes by antagonizing microRNAs ( miRNAs or miRs). However, the role of circRNA hsa_circ_0000092 in hepatocellular carcinoma (HCC) still remains enigmatic. Therefore, we aimed to investigate the specific mechanism of hsa_circ_0000092 in HCC. Differentially expressed circRNAs associated to HCC were initially analysed. The expression of hsa_circ_0000092, miR-338-3p and HN1 in HCC tissues and cell lines was examined. Next, the interaction among hsa_circ_0000092, miR-338-3p and HN1 was determined by dual- luciferase reporter, RNA pull-down and northern blot assays. Subsequently, a series of mimic, inhibitor or siRNA plasmids were delivered into HCC cells to validate the effects of hsa_circ_0000092, miR-338-3p and HN1 in controlling cell proliferation, migration, invasion and angiogenesis in vitro. Furthermore, the role of hsa_circ_0000092 in tumour growth of HCC in vivo was assessed with hsa_circ_0000092 depleted with siRNA. The hsa_circ_0000092/miR-338-3p/HN1 axis was predicted to participate in the development of HCC. hsa_circ_0000092 and HN1 were highly expressed while miR-338-3p was poorly expressed in HCC tissues and cell lines. hsa_circ_0000092 could competitively bind to miR-338-3p to up-regulate HN1 expression. Moreover, depleted hsa_circ_0000092 or elevated miR-338-3p was shown to suppress HCC cell proliferation, migration, invasion and angiogenesis in vitro via down-regulation of HN1. Furthermore, silencing hsa_circ_0000092 was demonstrated to suppress tumour growth in HCC in vivo. The results of this study suggested that hsa_circ_0000092 impaired miR-338-3p-mediated HN1 inhibition to aggravate the development of HCC, indicating that hsa_circ_0000092 is a potential candidate marker and therapeutic target for HCC.
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Authors | Jian Pu, Jianchu Wang, Wenchuan Li, Yuan Lu, Xianjian Wu, Xidai Long, Chunying Luo, Huamei Wei |
Journal | Journal of cellular and molecular medicine
(J Cell Mol Med)
Vol. 28
Issue 6
Pg. e15010
(Mar 2024)
ISSN: 1582-4934 [Electronic] England |
PMID | 32077624
(Publication Type: Journal Article)
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Copyright | © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. |
Chemical References |
- RNA, Circular
- MicroRNAs
- RNA, Small Interfering
- MIRN338 microRNA, human
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Topics |
- Humans
- Carcinoma, Hepatocellular
(genetics)
- RNA, Circular
(genetics)
- Liver Neoplasms
(genetics)
- MicroRNAs
(genetics)
- Cell Proliferation
(genetics)
- RNA, Small Interfering
- Gene Expression Regulation, Neoplastic
- Cell Line, Tumor
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