Potential immune regulatory role of miR-146a upon Aeromonas hydrophila and Edwardsiella piscicida infections in zebrafish.

Abstract

This study was aimed to understand the expression of miR-146a in zebrafish (Danio rerio) and its role in regulating immune responses during Aeromonas hydrophila and Edwardsiella piscicida infections. The miR-146a expression was observed from the 1-h post fertilization (hpf) stage and gradually increased up to the early larval stage of zebrafish. The ubiquitous expression of miR-146a was detected in all tested tissues, with the highest level in gills. The expression of miR-146a was significantly increased in larvae when exposed to E. piscicida infection at 24 and 48 h post exposure (hpe). Intraperitoneally (i.p.) injected A. hydrophila and E. piscicida into adult zebrafish showed significant upregulation of miR-146a in gills. Furthermore, immune-related genes, toll-like receptor, tlr-4, transducing signaling pathway molecules, traf-6 and myd88 (bacteria-infected larvae and adults), transcription factor relA and mcp-1b (bacteria-infected adults), pro-inflammatory, il-6 (A. hydrophila-exposed larvae) and mmp-9 (bacteria-exposed larvae) were significantly repressed. In contrast, il-1β, tnf-α, cxcl-18b, and ccl-34a.4 were induced in both bacteria-challenged larvae and adults. Based on the results, it is suggested that endogenous miR-146a could act as an infection inducible miRNA in zebrafish upon A. hydrophila and E. piscicida infections; also, it could potentially regulate the immune responses in zebrafish.

• Authors: T D Liyanage, Chamilani Nikapitiya, Jehee Lee, Mahanama De Zoysa
• Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (Braz J Microbiol) Vol. 51 Issue 3 Pg. 931-937 (Sep 2020) ISSN: 1678-4405 [Electronic] Brazil
• PMID: 32067211 (Publication Type: Journal Article)

Chemical References

• MIRN146 microRNA, zebrafish
• MicroRNAs

Topics

• Aeromonas hydrophila (physiology)
• Animals
• Edwardsiella (physiology)
• Fish Diseases (genetics, immunology, microbiology)
• Gene Expression Regulation
• Gram-Negative Bacterial Infections (genetics, immunology, microbiology, veterinary)
• Immunity (genetics)
• Life Cycle Stages (genetics)
• MicroRNAs (genetics, immunology)
• Zebrafish