Secreted protein acidic and rich in cysteine (SPARC) regulates thermogenesis in white and brown adipocytes.

Abstract

SPARC, also known as osteonectin, is well known for its physiological roles in bone formation and tissue remodeling, as well as in cancer pathology; however, evidence regarding its function in adipocytes is lacking. The present study explored the physiological role of SPARC in cultured 3T3-L1 white and HIB1B brown adipocytes of murine cell lines. Treatment of recombinant SPARC upregulated the fat browning marker proteins and genes in white adipocytes and activated brown adipocytes. Conversely, knockdown of Sparc markedly reduced these genes and proteins in both cell lines. In addition, recombinant SPARC inhibited expression of adipogenic and lipogenic proteins but elevated lipolytic and fatty acid oxidation proteins. Furthermore, in silico analysis revealed that SPARC directly interacted and regulated VEGF in adipocytes. In conclusion, SPARC acts as a regulatory protein in both white and brown adipocytes by controlling thermogenesis and is thus regarded as a possible therapeutic target for treatment of obesity.

• Authors: Sulagna Mukherjee, Min Ji Choi, Sang Woo Kim, Jong Won Yun
• Journal: Molecular and cellular endocrinology (Mol Cell Endocrinol) Vol. 506 Pg. 110757 (04 15 2020) ISSN: 1872-8057 [Electronic] Ireland
• PMID: 32057945 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Osteonectin
• Recombinant Proteins
• SPARC protein, mouse

Topics

• 3T3-L1 Cells
• Adipocytes, Brown (drug effects, physiology)
• Adipocytes, White (drug effects, physiology)
• Adipogenesis (drug effects, genetics)
• Animals
• Cell Transdifferentiation (drug effects)
• Cells, Cultured
• Lipid Metabolism (drug effects, genetics)
• Lipogenesis (drug effects, genetics)
• Mice
• Osteonectin (pharmacology, physiology)
• Recombinant Proteins (pharmacology)
• Thermogenesis (drug effects, genetics)