Application of antigen presenting cell-targeted nanovaccine delivery system in rhabdovirus disease prophylactics using fish as a model organism.

Abstract

BACKGROUND:

Targeted delivery of virus-associated antigens to professional antigen-presenting cells (APCs) is considered as an efficient strategy to enhance the pyrophytic effect of vaccines against rhabdovirus disease.

MATERIALS AND METHODS:

In this study, we constructed a targeted carbon nanotubes-based vaccine deliver system (SWCNTs-MG) which can recognize the signature receptor (mannose) of APCs. An environmentally and economically important disease called spring viremia of carp (SVC) was studied as a model to evaluate the feasibility of single-walled carbon nanotubes (SWCNTs) conjugated with mannosylated antigen for rhabdovirus prevention.

RESULTS:

Results showed that SWCNTs-MG could cross into fish body and present to internal immune-related tissues through gill, muscle and intestine within 6 h immersed vaccination. With further modification of mannose moiety, the obtained nanovaccine showed enhanced uptake by carp macrophages and immune-related tissues, which would then trigger strong immune responses against spring viremia of carp virus (SVCV) infection. Moreover, the survival rate of fish vaccinated with SWCNTs-MG (30 mg/L) was 63.5% after SVCV infection, whereas it was 0% for the control group.

CONCLUSION:

This study not only provide a theoretical basis and research template for the application of targeted nanovaccine system in aquatic animals, but also play an important role in supporting development of healthy aquaculture and ensuring the safety of aquatic products and ecology.

• Authors: Chen Zhang, Gao-Xue Wang, Bin Zhu
• Journal: Journal of nanobiotechnology (J Nanobiotechnology) Vol. 18 Issue 1 Pg. 24 (Jan 30 2020) ISSN: 1477-3155 [Electronic] England
• PMID: 32000788 (Publication Type: Journal Article)

Chemical References

• Drug Carriers
• Nanotubes, Carbon
• Vaccines, DNA
• Mannose

Topics

• Animals
• Antigen-Presenting Cells (metabolism)
• Carps
• Cell Membrane Permeability
• Drug Carriers (chemistry)
• Drug Liberation
• Fish Diseases (epidemiology, virology)
• Humans
• Immunization
• Macrophages (metabolism)
• Mannose (metabolism)
• Molecular Targeted Therapy
• Nanotubes, Carbon (chemistry)
• Rhabdoviridae (drug effects)
• Rhabdoviridae Infections (prevention & control, virology)
• Tissue Distribution
• Vaccination
• Vaccines, DNA (metabolism)