Differences in biological activities between recombinant human paraoxonase 1 (rhPON1) subtype isozemys R/Q as antidotes against organophosphorus poisonings.

Abstract

Paraoxonase 1 (PON1) is a type of aromatic esterase widely existing in mammals. It can hydrolyze various kinds of compounds effectively in vivo and in vitro. Previous studies have confirmed that PON1 can be used as antidote against organophosphorus poisonings (OPs). In this study, we obtained two subtype isozymes (i.e. rhPON1R192 and rhPON1Q192) by gene recombination and compared their detoxification effects against different OPs in rats. The rhPON1R192 demonstrated better detoxification effect against chlorpyrifos poisoning than the rhPON1Q192, whose detoxification effect against diazinon poisoning was prior to the former. Both of them showed poor detoxification effect against trithion. Therefore, we concluded that, to different OPs, better detoxification effect may be achieved by selecting the PON1 subtype isozyme with higher specific hydrolytic activity.

• Authors: Yue Cui, Min Zhao, Lang Han
• Journal: Toxicology letters (Toxicol Lett) Vol. 325 Pg. 51-61 (Jun 01 2020) ISSN: 1879-3169 [Electronic] Netherlands
• PMID: 31981688 (Publication Type: Journal Article)
• Copyright: Copyright © 2020. Published by Elsevier B.V.

Chemical References

• Antidotes
• Cholinesterase Inhibitors
• Isoenzymes
• Organothiophosphorus Compounds
• Recombinant Proteins
• carbophenothion
• Acetylcholinesterase
• Aryldialkylphosphatase
• PON1 protein, human
• Chlorpyrifos
• Diazinon

Topics

• Acetylcholinesterase (metabolism)
• Animals
• Antidotes (chemistry, pharmacology)
• Aryldialkylphosphatase (chemistry, pharmacology)
• Brain (pathology)
• Chlorpyrifos
• Cholinesterase Inhibitors (pharmacology)
• Diazinon
• Humans
• Isoenzymes (chemistry, pharmacology)
• Lethal Dose 50
• Male
• Organophosphate Poisoning (drug therapy, pathology)
• Organothiophosphorus Compounds (poisoning)
• Rats
• Rats, Wistar
• Recombinant Proteins (chemistry, pharmacology)