Abstract |
We previously reported that Momordin Ic, a natural triterpenoid saponin from the fruit of Kochia scoparia (L.) Schrad., exerts good anti-invasive activity on liver cancer partly by altering E-cadherin, VCAM-1, ICAM-1 and MMP-9. The JNK and p38-MAPK pathways differentially altered the four molecules to some extent. However, MMP-9, which is greatly suppressed by Momordin Ic, was affected by neither p38-MAPK nor JNK. Therefore, we further investigated how other signals previously found to regulate cell growth, such as COX-2 and PPARγ, function in the process of cell invasion by western blot. The results demonstrated that COX-2 and PPARγ play a significant role in Momordin Ic-inhibited cell invasion. However, COX-2 only regulated E-cadherin and ICAM-1. PPARγ was not involved in VCAM-1alteration but was significant for the expressions of other proteins. Akt, a kinase upstream of COX-2 and PPARγ, did not influence ICAM-1 but directly mediated the expression of E-cadherin, VCAM-1 and MMP-9. Momordin Ic weakens HepG2 cell invasion through PPARγ activation and COX-2 inhibition. These findings provide evidence for the anti-invasion mechanism of Momordin Ic.
|
Authors | Jing Wang, Ying Han, Man Wang, Qianqian Zhao, Xuefeng Chen, Xuebo Liu |
Journal | Toxicology in vitro : an international journal published in association with BIBRA
(Toxicol In Vitro)
Vol. 65
Pg. 104784
(Jun 2020)
ISSN: 1879-3177 [Electronic] England |
PMID | 31981603
(Publication Type: Journal Article)
|
Copyright | Copyright © 2020. Published by Elsevier Ltd. |
Chemical References |
- Antineoplastic Agents
- Cyclooxygenase 2 Inhibitors
- PPAR gamma
- Plant Extracts
- Saponins
- Triterpenes
- momordin
|
Topics |
- Antineoplastic Agents
(pharmacology)
- Cell Movement
(drug effects)
- Cyclooxygenase 2 Inhibitors
(pharmacology)
- Hep G2 Cells
- Humans
- PPAR gamma
(metabolism)
- Plant Extracts
(pharmacology)
- Saponins
(pharmacology)
- Triterpenes
(pharmacology)
|