The Lipoxin A4 Receptor Agonist BML-111 Alleviates Inflammatory Injury and Oxidative Stress in Spinal Cord Injury.

Abstract

BACKGROUND Spinal cord injury (SCI) has a high incidence and causes serious harm. Lipoxin A4 (LXA4) receptor agonist BML-111 was reported to regulate inflammation and oxidative stress. The goal of this study was to assess whether BML-111 could protect against SCI by suppressing inflammation and oxidative stress. MATERIAL AND METHODS We developed a rat SCI model, then BML-111 was intraperitoneally injected into SCI rats to observe the BML-111 function. The pathological changes of SCI were observed with hematoxylin and eosin (HE) staining. Motor function of rats were assessed by the modified Tarlov's scale. ELISA was used to assess the changes in levels of TNF-alpha, IL-1ß, and IL-6. Western blot analysis was performed to assess the expressions of TNF-alpha, IL-1ß, IL-6, Bcl2, Bax, and cleaved caspase3 in spinal cord tissue. TOS and TAS in rat serum were detected by xylenol orange method and ABTS method, respectively. The apoptotic cells in spinal cord tissue were observed with TUNEL assay. RESULTS The results indicated that BML-111 effectively improved the SCI and motor function of rats. BML-111 treatment decreased the levels of TNF-alpha, IL-1ß, and IL-6 in serum and spinal cord tissue, as well as decreasing the levels of TOS and TAS and cell apoptosis. CONCLUSIONS BML-111 alleviated inflammation and oxidative stress in SCI rats.

• Authors: Jian Liu, Lei Peng, Jie Li
• Journal: Medical science monitor : international medical journal of experimental and clinical research (Med Sci Monit) Vol. 26 Pg. e919883 (Jan 23 2020) ISSN: 1643-3750 [Electronic] United States
• PMID: 31971927 (Publication Type: Journal Article)

Chemical References

• 5(S),6(R)-7-trihydroxyheptanoic acid, methyl ester
• Heptanoic Acids
• Interleukin-1beta
• Interleukin-6
• Lipoxins
• Protective Agents
• Receptors, Lipoxin
• Tumor Necrosis Factor-alpha
• lipoxin A(4) receptor, rat
• lipoxin A4

Topics

• Animals
• Apoptosis (drug effects)
• Heptanoic Acids (metabolism, pharmacology)
• Inflammation (drug therapy)
• Interleukin-1beta
• Interleukin-6
• Lipoxins (metabolism)
• Male
• Oxidative Stress (drug effects, physiology)
• Protective Agents (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Receptors, Lipoxin (agonists, metabolism)
• Spinal Cord (pathology)
• Spinal Cord Injuries (drug therapy, pathology)
• Tumor Necrosis Factor-alpha