Four recombinant (r)
allergens (rAmb a 8.0101, rArt v 4.0101, rBet v 2.0101, and rPhl p 12.0101) were successfully produced and used for sensitization studies. The
allergens belong to the
profilin family which is one of the most numerous
allergen families. These four
proteins represent
allergens originating from pollen of weeds (rAmb a 8.0101 and rArt v 4.0101), tree (rBet v 2.0101) and grass (rPhl p 12.0101). The recombinant
allergens were characterized using various biochemical and biophysical methods and tested for their ability to bind patient-derived
antibodies. One hundred patients aged 2 to 50 years sensitized to pollen and plant-derived food
allergens (
IgE > 0.35 kU/L) were included. Sensitization to individual
allergen sources and components of birch and timothy pollens was evaluated using multiparameter immunoblots. The presence of
IgE to pollen-derived recombinant
profilins rAmb a 8.0101, rArt v 4.0101, rBet v 2.0101, and rPhl p 12.0101 in serum was evaluated using ELISA method. The presence of
IgE against pollen
profilins was detected in 20 out of 100 studied patients. High correlation was seen between
IgE ELISA results with individual pollen
profilins. In summary, it was shown that the recombinant versions of the four allergenic
profilins can be used for sensitization studies and for component-resolved
allergy diagnostics.